Carreno R A, Martin D S, Barta J R
Department of Pathobiology, Ontario Veterinary College, University Guelph, Canada.
Parasitol Res. 1999 Nov;85(11):899-904. doi: 10.1007/s004360050655.
The phylogenetic placement of gregarine parasites (Apicomplexa: Gregarinasina) within the Apicomplexa was derived by comparison of small-subunit ribosomal RNA gene sequences. Gregarine sequences were obtained from Gregarina niphandrodes Clopton, Percival, and Janovy, 1991, and Monocystis agilis Stein, 1848 (Eugregarinorida Léger 1900), as well as from Ophriocystis elektroscirrha McLaughlin and Myers, 1970 (Neogregarinorida Grassé 1953). The sequences were aligned with several other gregarine and apicomplexan sequences from GenBank and the resulting data matrix analyzed by parsimony and maximum-likelihood methods. The gregarines form a monophyletic clade that is a sister group to Cryptosporidium spp. The gregarine/ Cryptosporidium clade is separate from the other major apicomplexan clade containing the coccidia, adeleids, piroplasms, and haemosporinids. The trees indicate that the genus Cryptosporidium has a closer phylogenetic affinity with the gregarines than with the coccidia. These results do not support the present classification of the Cryptosporidiidae in the suborder Eimerioirina Léger, 1911.
通过比较小亚基核糖体RNA基因序列，得出了簇虫寄生虫（顶复门：簇虫亚纲）在顶复门中的系统发育位置。簇虫序列取自1991年的尼氏簇虫（Gregarina niphandrodes）、克洛普顿、珀西瓦尔和贾诺维，以及1848年的敏捷单囊簇虫（Monocystis agilis）（真簇虫目，勒热尔，1900年），还有1970年的麦氏蜂簇虫（Ophriocystis elektroscirrha）（麦克劳克林和迈尔斯）（新簇虫目，格拉塞，1953年）。这些序列与来自GenBank的其他几种簇虫和顶复门生物的序列进行了比对，并通过简约法和最大似然法对所得数据矩阵进行了分析。簇虫形成了一个单系类群，是隐孢子虫属的姐妹群。簇虫/隐孢子虫类群与包含球虫、艾美球虫、梨形虫和血孢子虫的其他主要顶复门类群分开。这些树状图表明，隐孢子虫属与簇虫的系统发育亲缘关系比与球虫更近。这些结果不支持目前将隐孢子虫科分类在艾美球虫亚目（勒热尔，1911年）中的分类方法。
