猪肠道病毒1型（PEV-1）塔尔凡毒株RNA依赖的RNA聚合酶（RdRp）的序列测定及系统发育分析

Sequence determination and phylogenetic analysis of RNA-dependent RNA polymerase (RdRp) of the porcine enterovirus 1 (PEV-1) Talfan strain.

作者信息

Kaku Y, Yamada S, Murakami Y

机构信息

Department of Virology, National Institute of Animal Health, Ibaraki, Japan.

出版信息

Arch Virol. 1999;144(9):1845-52. doi: 10.1007/s007050050709.

DOI:10.1007/s007050050709

PMID:10542031

Abstract

The nucleotide sequence of the region including the RNA-dependent RNA polymerase (RdRp) of the porcine enterovirus 1 (PEV-1) Talfan strain was determined. Amino acid identities of Talfan RdRp with those of other picornaviruses were significantly lower than those seen among the viruses of the same genus of picornaviruses. The phylogenetic analysis of RdRp indicated that picornaviruses were divided into 8 clusters, in which Talfan was genetically distinct from other picornaviruses in contrast to the current taxonomy. In this study, we propose that PEV-1 Talfan be regarded as a new genus for the family Picorna- viridae.

摘要

测定了猪肠道病毒1型（PEV-1）塔尔凡毒株包括依赖RNA的RNA聚合酶（RdRp）区域的核苷酸序列。塔尔凡RdRp与其他小RNA病毒的氨基酸同一性显著低于同一属小RNA病毒之间的同一性。RdRp的系统发育分析表明，小RNA病毒分为8个簇，其中塔尔凡在遗传上与其他小RNA病毒不同，这与当前的分类法不同。在本研究中，我们建议将PEV-1塔尔凡视为小RNA病毒科的一个新属。