匈牙利野猪中一种新型猪肠道病毒的特性研究。
Characterization of a novel porcine enterovirus in wild boars in Hungary.
机构信息
Regional Laboratory of Virology, National Reference Laboratory of Gastroenteric Viruses, ÁNTSZ Regional Institute of State Public Health Service, Szabadság út 7, Pecs 7623, Hungary.
出版信息
Arch Virol. 2012 May;157(5):981-6. doi: 10.1007/s00705-012-1255-5. Epub 2012 Feb 15.
Abstract
Porcine enteroviruses (PEVs) are members of the family Picornaviridae, genus Enterovirus. Until now, only three different PEV genotypes (PEV-9 and -10, and PEV-3H/PEV-14) have been detected in domestic pigs, and there is no information about the presence of PEVs in wild animals. Here, we identify and characterize the complete genomes of PEV originated from 5 of 10 (50%) of wild boar (Sus scrofa) piglets by RT-PCR and pyrosequencing. Wild boar/WBD/2011/HUN (JN807387) PEV showed only 67% amino acid identity in VP1 compared to the most closely related prototype PEV-3H/PEV-14. Wild boar enterovirus represents a novel PEV genotype, provisionally called PEV-15.
摘要
猪肠道病毒（PEVs）属于小核糖核酸病毒科肠道病毒属。到目前为止，仅在国内猪中检测到三种不同的 PEV 基因型（PEV-9 和 -10 以及 PEV-3H/PEV-14），而在野生动物中尚无关于 PEV 存在的信息。在这里，我们通过 RT-PCR 和焦磷酸测序，从 10 只（50%）野猪仔猪中鉴定并表征了源自 5 只（50%）野猪仔猪的 PEV 的完整基因组。野猪/WBD/2011/HUN（JN807387）PEV 在 VP1 中与最密切相关的原型 PEV-3H/PEV-14 的氨基酸同一性仅为 67%。野猪肠道病毒代表了一种新型的 PEV 基因型，暂命名为 PEV-15。
相似文献
1
Characterization of a novel porcine enterovirus in wild boars in Hungary.匈牙利野猪中一种新型猪肠道病毒的特性研究。
Arch Virol. 2012 May;157(5):981-6. doi: 10.1007/s00705-012-1255-5. Epub 2012 Feb 15.
2
Characterization of a novel porcine enterovirus in domestic pig in Hungary.匈牙利国内猪中一种新型猪肠道病毒的特性研究。
Infect Genet Evol. 2011 Jul;11(5):1096-102. doi: 10.1016/j.meegid.2011.04.003. Epub 2011 Apr 12.
3
Complete genome sequence of a novel porcine enterovirus strain in China.中国一株新型猪肠道病毒的全基因组序列。
J Virol. 2012 Jun;86(12):7008-9. doi: 10.1128/JVI.00711-12.
4
Detection of porcine enteroviruses by nRT-PCR: differentiation of CPE groups I-III with specific primer sets.通过逆转录巢式聚合酶链反应检测猪肠道病毒：用特异性引物组区分I-III组细胞病变效应
J Virol Methods. 2000 Aug;88(2):205-18. doi: 10.1016/s0166-0934(00)00189-0.
5
Porcine teschovirus in wild boars in Hungary.匈牙利野猪中的猪细小病毒。
Arch Virol. 2012 Aug;157(8):1573-8. doi: 10.1007/s00705-012-1327-6. Epub 2012 May 9.
6
Prevalence of porcine enterovirus 9 in pigs in middle and eastern China.中国中部和东部地区猪中肠病毒 9 的流行情况。
Virol J. 2013 Mar 28;10:99. doi: 10.1186/1743-422X-10-99.
7
Natural interspecies recombinant bovine/porcine enterovirus in sheep.绵羊中的天然种间重组牛/猪肠道病毒。
J Gen Virol. 2012 Sep;93(Pt 9):1941-1951. doi: 10.1099/vir.0.041335-0. Epub 2012 May 30.
8
Porcine kobuvirus in wild boars (Sus scrofa).野猪中的猪博卡病毒（Sus scrofa）。
Arch Virol. 2013 Jan;158(1):281-2. doi: 10.1007/s00705-012-1456-y. Epub 2012 Aug 29.
9
The survey of porcine teschoviruses, sapeloviruses and enteroviruses B infecting domestic pigs and wild boars in the Czech Republic between 2005 and 2011.2005 年至 2011 年期间，对捷克共和国的家猪和野猪进行了肠病毒 B、口疮病毒和肠道病毒的调查。
Infect Genet Evol. 2012 Oct;12(7):1447-51. doi: 10.1016/j.meegid.2012.04.025. Epub 2012 May 3.
10
Prevalence, genetic diversity and recombination of species G enteroviruses infecting pigs in Vietnam.越南猪源肠道病毒 G 型的流行、遗传多样性和重组情况。
J Gen Virol. 2014 Mar;95(Pt 3):549-556. doi: 10.1099/vir.0.061978-0. Epub 2013 Dec 9.
引用本文的文献
1
Construction of enterovirus G expressing reporter genes for antiviral drug screening assays.构建用于抗病毒药物筛选试验的表达报告基因的肠道病毒G
BMC Vet Res. 2025 Aug 13;21(1):515. doi: 10.1186/s12917-025-04960-0.
2
First isolation, identification, and pathogenicity evaluation of an EV-G6 strain in China.中国首次对一株肠道病毒G6型毒株进行分离、鉴定及致病性评估。
Front Vet Sci. 2024 Jul 24;11:1431180. doi: 10.3389/fvets.2024.1431180. eCollection 2024.
3
Distribution and genetic diversity of Enterovirus G (EV-G) on pig farms in Thailand.泰国猪场肠道病毒 G（EV-G）的分布与遗传多样性。
BMC Vet Res. 2021 Aug 16;17(1):277. doi: 10.1186/s12917-021-02988-6.
4
Isolation, Identification, and Evaluation of the Pathogenicity of a Porcine Enterovirus G Isolated From China.从中国分离出的猪肠道病毒G的分离、鉴定及致病性评估
Front Vet Sci. 2021 Jul 22;8:712679. doi: 10.3389/fvets.2021.712679. eCollection 2021.
5
Teschovirus and other swine and human enteric viruses in Brazilian watersheds impacted by swine husbandry.巴西受养猪业影响的流域中的口蹄疫病毒和其他猪及人类肠道病毒。
Braz J Microbiol. 2020 Jun;51(2):711-717. doi: 10.1007/s42770-019-00197-w. Epub 2019 Nov 30.
6
Molecular Identification of Enteroviruses from Cattle and Goat Feces and Environment in Thailand.泰国牛和山羊粪便及环境中肠道病毒的分子鉴定。
Appl Environ Microbiol. 2019 Feb 20;85(5). doi: 10.1128/AEM.02420-18. Print 2019 Mar 1.
7
First detection of novel enterovirus G recombining a torovirus papain-like protease gene associated with diarrhoea in swine in South Korea.首次在韩国猪群中发现与腹泻相关的新型肠道病毒 G 重组了冠状病毒木瓜蛋白酶样蛋白酶基因。
Transbound Emerg Dis. 2019 Mar;66(2):1023-1028. doi: 10.1111/tbed.13073. Epub 2018 Dec 1.
8
Genetic diversity and recombination of enterovirus G strains in Japanese pigs: High prevalence of strains carrying a papain-like cysteine protease sequence in the enterovirus G population.日本猪中肠道病毒G株的遗传多样性与重组：肠道病毒G群体中携带类木瓜蛋白酶半胱氨酸蛋白酶序列毒株的高流行率
PLoS One. 2018 Jan 11;13(1):e0190819. doi: 10.1371/journal.pone.0190819. eCollection 2018.
9
Identification of an enterovirus recombinant with a torovirus-like gene insertion during a diarrhea outbreak in fattening pigs.在育肥猪腹泻疫情期间鉴定出一种插入类环曲病毒基因的肠道病毒重组体。
Virus Evol. 2017 Sep 8;3(2):vex024. doi: 10.1093/ve/vex024. eCollection 2017 Jul.
10
A porcine enterovirus G associated with enteric disease contains a novel papain-like cysteine protease.一种与肠道疾病相关的猪肠道病毒G含有一种新型木瓜蛋白酶样半胱氨酸蛋白酶。
J Gen Virol. 2017 Jun;98(6):1305-1310. doi: 10.1099/jgv.0.000799. Epub 2017 Jun 8.
本文引用的文献
1
Analyzing the genetic diversity of teschoviruses in Spanish pig populations using complete VP1 sequences.利用完整 VP1 序列分析西班牙猪群中的口蹄疫病毒遗传多样性。
Infect Genet Evol. 2011 Dec;11(8):2144-50. doi: 10.1016/j.meegid.2011.09.014. Epub 2011 Sep 29.
2
MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods.MEGA5：用于最大似然法、进化距离法和最大简约法的分子进化遗传学分析。
Mol Biol Evol. 2011 Oct;28(10):2731-9. doi: 10.1093/molbev/msr121. Epub 2011 May 4.
3
Characterization of a novel porcine enterovirus in domestic pig in Hungary.匈牙利国内猪中一种新型猪肠道病毒的特性研究。
Infect Genet Evol. 2011 Jul;11(5):1096-102. doi: 10.1016/j.meegid.2011.04.003. Epub 2011 Apr 12.
4
Molecular characterization and phylogenetic analysis of VP1 of porcine enteric picornaviruses isolates in Italy.意大利肠型猪微小 RNA 病毒分离株 VP1 的分子特征和系统进化分析。
Transbound Emerg Dis. 2010 Dec;57(6):434-42. doi: 10.1111/j.1865-1682.2010.01170.x. Epub 2010 Oct 8.
5
RDP3: a flexible and fast computer program for analyzing recombination.RDP3：一个灵活快速的计算机程序，用于分析重组。
Bioinformatics. 2010 Oct 1;26(19):2462-3. doi: 10.1093/bioinformatics/btq467. Epub 2010 Aug 26.
6
Wild boars as sources for infectious diseases in livestock and humans.野猪作为家畜和人类传染病的传染源。
Philos Trans R Soc Lond B Biol Sci. 2009 Sep 27;364(1530):2697-707. doi: 10.1098/rstb.2009.0086.
7
Metagenomic analyses of viruses in stool samples from children with acute flaccid paralysis.急性弛缓性麻痹患儿粪便样本中病毒的宏基因组分析。
J Virol. 2009 May;83(9):4642-51. doi: 10.1128/JVI.02301-08. Epub 2009 Feb 11.
8
A highly prevalent and genetically diversified Picornaviridae genus in South Asian children.南亚儿童中一种高度流行且基因多样化的小RNA病毒科属。
Proc Natl Acad Sci U S A. 2008 Dec 23;105(51):20482-7. doi: 10.1073/pnas.0807979105. Epub 2008 Nov 25.
9
Sequencing of porcine enterovirus groups II and III reveals unique features of both virus groups.猪肠道病毒II组和III组的测序揭示了这两个病毒组的独特特征。
J Virol. 2002 Jun;76(11):5813-21. doi: 10.1128/jvi.76.11.5813-5821.2002.
10
Genetic reclassification of porcine enteroviruses.猪肠道病毒的基因重新分类
J Gen Virol. 2001 Feb;82(Pt 2):417-424. doi: 10.1099/0022-1317-82-2-417.
Zotero 插件