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[相差显微镜技术与免疫荧光技术在猪痢疾实验室诊断中的适用性比较]

[Comparison of the suitability of phase-contrast and immunofluorescence technics in the laboratory diagnosis of porcine dysentery].

作者信息

Hornich M, Chrástová V

出版信息

Vet Med (Praha). 1979 Jan;24(1):29-36.

PMID:105449
Abstract

Immunofluorescence technique, compared with the method of phase contrast, does not appear to be better for laboratory diagnostics of swine dysentery because neither of these methods can be used for distinguishing between pathogenic and nonpathogenic strains of treponemas. The number of treponemas contained in faeces should still be considered to be the main criterion in laboratory diagnostics. In clinically healthy pigs from stocks which never suffered from dysentery treponemas were found only in few cases and always in small numbers. The numbers of treponemas contained in the faeces of dysenteric pigs were several times higher. Antigenic relationship of one nonpathogenic and three pathogenic strains of Treponema hyodysenteriae was proved by the agglutination and fluoresence methods.

摘要

与相差显微镜法相比,免疫荧光技术对于猪痢疾的实验室诊断似乎并无优势,因为这两种方法均无法用于区分致病性和非致病性密螺旋体菌株。粪便中密螺旋体的数量仍应被视为实验室诊断的主要标准。在从未患过痢疾的猪群中,临床健康猪仅在少数情况下发现有密螺旋体,且数量总是很少。患痢疾猪粪便中的密螺旋体数量要高出几倍。通过凝集和荧光方法证实了一株非致病性猪痢疾密螺旋体和三株致病性猪痢疾密螺旋体之间的抗原关系。

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