Sobell H M
Proc Natl Acad Sci U S A. 1975 Jan;72(1):279-83. doi: 10.1073/pnas.72.1.279.
A mechanism to activate branch migration between homologous DNA molecules is described that leads to synapsis in genetic recombination. The model involves a restriction-like endonucleolytic enzyme that first nicks DNA (to produce single-strand breaks) on strands of opposite polarity at symmetrically arranged nucleotide sequences (located at ends of genes or operons). This is followed by local denaturation of the region, promoted by a single-strand-specific DNA binding protein (i.e., an unwinding protein). Hydrogen-bounding between homologous DNA molecules can then be initiated and this allows for subsequent propagation of hybrid DNA in the pathway to formation of the synapton structure.
描述了一种激活同源DNA分子间分支迁移的机制,该机制导致基因重组中的联会。该模型涉及一种类似限制酶的核酸内切酶,它首先在对称排列的核苷酸序列(位于基因或操纵子末端)处,在相反极性的链上切割DNA(产生单链断裂)。随后,由单链特异性DNA结合蛋白(即解旋蛋白)促进该区域的局部变性。然后可以启动同源DNA分子之间的氢键结合,这允许杂种DNA在形成联会结构的途径中随后传播。