基因重组：RecA蛋白促进双链DNA分子间的同源配对，而无需解链。
Genetic recombination: recA protein promotes homologous pairing between duplex DNA molecules without strand unwinding.
作者信息
Cassuto E, West S C, Podell J, Howard-Flanders P
出版信息
Nucleic Acids Res. 1981 Aug 25;9(16):4201-10. doi: 10.1093/nar/9.16.4201.
Abstract
The recA protein of Escherichia coli promotes pairing in vitro between covalent circular duplex DNA and homologous circular duplex DNA containing a single stranded region. We have used a filter binding assay to investigate the frequency of homologous pairing between gapped and intact duplex DNA when unwinding of the free 3' and 5' ends of the gapped molecules was blocked. In order to obtain DNA without free ends, the gapped DNA was treated with trimethylpsoralen and 360 nm light so as to introduce about 6 crosslinks per DNA molecule and the double stranded regions on either side of the gaps were then digested up to the first crosslinks with exonuclease III and lambda exonuclease. This treatment did not diminish the frequency of homologous pairing, an observation which is difficult to reconcile with models for recombination requiring strand unwinding before pairing.
摘要
大肠杆菌的RecA蛋白在体外可促进共价闭合双链DNA与含有单链区域的同源环状双链DNA之间的配对。我们采用滤膜结合试验来研究当缺口分子的游离3'端和5'端的解旋被阻断时，缺口双链DNA与完整双链DNA之间同源配对的频率。为了获得无游离端的DNA，用三甲基补骨脂素和360nm光处理缺口DNA，以便每个DNA分子引入约6个交联，然后用核酸外切酶III和λ核酸外切酶将缺口两侧的双链区域消化至第一个交联处。这种处理并没有降低同源配对的频率，这一观察结果难以与那些认为在配对前需要链解旋的重组模型相协调。
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本文引用的文献
1
Homologous pairing in genetic recombination: recA protein makes joint molecules of gapped circular DNA and closed circular DNA.遗传重组中的同源配对：RecA蛋白使缺口环状DNA和闭环状DNA形成联合分子。
Cell. 1980 May;20(1):223-35. doi: 10.1016/0092-8674(80)90250-0.
2
recA protein promotes homologous-pairing and strand-exchange reactions between duplex DNA molecules.RecA蛋白促进双链DNA分子之间的同源配对和链交换反应。
Proc Natl Acad Sci U S A. 1981 Apr;78(4):2100-4. doi: 10.1073/pnas.78.4.2100.
3
Homologous pairing can occur before DNA strand separation in general genetic recombination.在一般的基因重组过程中，同源配对可在DNA链分离之前发生。
Nature. 1981 Mar 5;290(5801):29-33. doi: 10.1038/290029a0.
4
Initiation of genetic recombination: homologous pairing between duplex DNA molecules promoted by recA protein.遗传重组的起始：由recA蛋白促进的双链DNA分子之间的同源配对。
Proc Natl Acad Sci U S A. 1980 Jul;77(7):3962-6. doi: 10.1073/pnas.77.7.3962.
5
Recognition of duplex DNA containing single-stranded regions by recA protein.RecA蛋白对含有单链区域的双链DNA的识别。
Proc Natl Acad Sci U S A. 1980 May;77(5):2569-73. doi: 10.1073/pnas.77.5.2569.
6
Specificity of the three-stranded complex formation between double-stranded DNA and single-stranded RNA containing repeating nucleotide sequences.双链DNA与含有重复核苷酸序列的单链RNA之间三链复合物形成的特异性。
J Mol Biol. 1968 Oct 14;37(1):63-80. doi: 10.1016/0022-2836(68)90073-9.
7
Models of specifically paired like (homologous) nucleic acid structures.特异性配对的类似（同源）核酸结构模型。
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