Hirano T, Kurono Y, Ichimiya I, Suzuki M, Mogi G
Department of Otolaryngology, Oita Medical University, Japan.
Otolaryngol Head Neck Surg. 1999 Nov;121(5):616-21. doi: 10.1016/S0194-5998(99)70068-9.
To clarify the role of viral infection in otitis media, we intranasally inoculated mice with influenza A virus and examined histologic changes in the nasopharyngeal mucosa using a battery of lectins. Additionally, live Haemophilus influenzae or Streptococcus pneumoniae was injected into the nasopharynx after virus inoculation, and the clearance of bacteria from the nasopharynx was examined. Staining of the mucous blanket and epithelial cell surfaces in the nasopharynx with peanut agglutinin, succinyl wheat-germ agglutinin, and Bandeiraea simplicifolia agglutinin was significantly enhanced with intranasal virus inoculation when compared with that in control animals. The nasopharynx was moderately stained with Maachia amurensis agglutinin and wheat-germ agglutinin in control animals, and the staining was enhanced after virus inoculation. These findings were most remarkable 5 and 9 days after virus inoculation. The numbers of bacteria cultured from the nasopharynx were significantly increased when bacteria were injected 5 days after virus inoculation. These results suggest that an alteration in the glycoconjugate structure lining the nasopharyngeal mucosa caused by the influenza virus might be associated with the reduction in bacterial clearance.
为阐明病毒感染在中耳炎中的作用，我们通过鼻腔给小鼠接种甲型流感病毒，并使用一系列凝集素检查鼻咽黏膜的组织学变化。此外，在病毒接种后将活的流感嗜血杆菌或肺炎链球菌注入鼻咽，并检查细菌从鼻咽的清除情况。与对照动物相比，经鼻腔接种病毒后，用花生凝集素、琥珀酰小麦胚芽凝集素和单叶豆凝集素对鼻咽部黏液层和上皮细胞表面进行染色，其染色显著增强。对照动物的鼻咽用龙牙楤木凝集素和小麦胚芽凝集素进行适度染色，病毒接种后染色增强。这些发现在病毒接种后5天和9天最为显著。当在病毒接种后5天注射细菌时，从鼻咽培养出的细菌数量显著增加。这些结果表明，流感病毒引起的鼻咽黏膜内衬糖缀合物结构的改变可能与细菌清除减少有关。
