Plotkowski M C, Puchelle E, Beck G, Jacquot J, Hannoun C
Am Rev Respir Dis. 1986 Nov;134(5):1040-4. doi: 10.1164/arrd.1986.134.5.1040.
Bacterial adherence to virus-infected respiratory tract cells may be one of the several mechanisms whereby virus predisposes to bacterial pneumonia. To evaluate the effect of influenza virus infection on pneumococcus adhesion, 39 mice were infected with PR8/A influenza virus. The adherence of radiolabeled pneumococcus to mice tracheal cells was determined 2, 4, and 6 days after viral inoculation. The pneumococcal adhesion to infected tracheas was significantly enhanced on Day 6 (p less than 0.001). Scanning and transmission electron microscopy revealed that by the fourth and sixth days after virus inoculation, the ciliated and the secretory cells of the tracheal epithelium had desquamated and the mucosa were coated with a continuous layer of basal cells. In a few cases, a desquamation of the basal layer was observed and the exposed basement membrane appeared as a pole of attraction for bacteria. Pneumococci were never seen attached to control tracheas. In contrast, they were observed adhered to the microvilli of the basal cells and, to a greater extent, to the exposed basement membrane.
细菌黏附于病毒感染的呼吸道细胞可能是病毒诱发细菌性肺炎的多种机制之一。为评估流感病毒感染对肺炎球菌黏附的影响，39只小鼠感染了PR8/A流感病毒。在病毒接种后第2、4和6天，测定放射性标记的肺炎球菌对小鼠气管细胞的黏附情况。在第6天，肺炎球菌对感染气管的黏附显著增强（p小于0.001）。扫描电子显微镜和透射电子显微镜显示，在病毒接种后的第4天和第6天，气管上皮的纤毛细胞和分泌细胞已脱落，黏膜被一层连续的基底细胞覆盖。在少数情况下，观察到基底层脱落，暴露的基底膜成为细菌的一个吸引点。在对照气管中从未见到肺炎球菌附着。相反，观察到它们附着于基底细胞的微绒毛上，并且在更大程度上附着于暴露的基底膜上。
