Bentley R, Bennett J W
Department of Biological Sciences, University of Pittsburgh, Pennsylvania 15260, USA.
Annu Rev Microbiol. 1999;53:411-46. doi: 10.1146/annurev.micro.53.1.411.
In a new golden age, polyketides are investigated and manipulated with the tools of molecular biology and genetics; hybrid polyketides can be produced. Pharmaceutical companies hope to find new and useful polyketide products, including antibiotics, anthelminthics, and immunosuppressants. This review describes the past developments (largely chemical) on which the present investigations are based, attempts to make sense of the expanding scope of polyketides, looks at the shifting research focus around polyketides, presents a working definition in biosynthetic terms, and takes note of recent work in combinatorial biosynthesis. Also discussed is the failure of the classical enzymological approach to polyketide biosynthesis.
在一个新的黄金时代，人们运用分子生物学和遗传学工具对聚酮化合物进行研究和操控，从而能够生产出杂合聚酮化合物。制药公司希望找到新的、有用的聚酮化合物产品，包括抗生素、驱虫药和免疫抑制剂。本综述描述了当前研究基于的过去的发展情况（主要是化学方面），试图理解聚酮化合物不断扩大的范围，审视围绕聚酮化合物的研究重点的转变，给出一个基于生物合成的实用定义，并关注组合生物合成方面的近期工作。还讨论了经典酶学方法在聚酮化合物生物合成研究中的失败。
