Ban J, Hlavaty J, Orlik O, Splitter G A, Altaner C
Cancer Research Institute of the Slovak Academy of Sciences, Bratislava, Slovakia.
Arch Virol. 1999;144(10):2013-22. doi: 10.1007/s007050050722.
The relationship between the putative bovine leukemia virus receptor gene (BVLRcp) and the susceptibility of human cells to BLV infection was studied. Three cDNA clones encoding different portions of the human equivalent of bovine BLVRcp1 were isolated by DNA-DNA hybridization by comparison of the human cDNA clones to bovine BLVRcp1. Amino acid sequence indicated that the human sequence encodes the delta subunit of the AP-3 adaptor-related protein. When the recombinant human homologue BLVRcp2 was expressed in E. coli, it failed to bind the BLVgp51. However, the BVLVgp51 binding ability was restored when the chimerical BLVRcp molecule was prepared by exchanging 5' ends between bovine and human BLVRcp cDNAs. This finding implies that this BLVgp51 binding site is present only on the bovine BLVRcp and therefore its human homologue cannot be recognized by BLVgp51. This might also explain the resistance of human cells to BLV infection.
研究了假定的牛白血病病毒受体基因（BVLRcp）与人类细胞对BLV感染易感性之间的关系。通过将人类cDNA克隆与牛BLVRcp1进行比较，利用DNA-DNA杂交分离出了三个编码人类等效物牛BLVRcp1不同部分的cDNA克隆。氨基酸序列表明，人类序列编码AP-3衔接蛋白相关蛋白的δ亚基。当重组人同源物BLVRcp2在大肠杆菌中表达时，它未能结合BLVgp51。然而，当通过在牛和人BLVRcp cDNA之间交换5'末端制备嵌合BLVRcp分子时，BVLVgp51的结合能力得以恢复。这一发现意味着该BLVgp51结合位点仅存在于牛BLVRcp上，因此其人类同源物不能被BLVgp51识别。这也可能解释了人类细胞对BLV感染的抗性。
