Suzuki T, Ikeda H
Laboratory of Immunogenetics, National Institute of Animal Health, Tsukuba, Ibaraki-ken, Japan.
J Virol. 1998 Jan;72(1):593-9. doi: 10.1128/JVI.72.1.593-599.1998.
A mouse cDNA (mBLVR1) which was highly homologous to the bovine cDNA of the bovine leukemia virus receptor (BLVR) gene was cloned. The mBLVR1 cDNA, of 4,730 bp, covered nearly the full length of the mRNA (about 5 kb) and included an open reading frame (ORF) encoding a protein of 1,199 amino acids. While the bovine BLVR protein was thought to be a type I transmembrane protein, the deduced protein coded by mBLVR1 did not appear to be a typical transmembrane protein. The ORF of mBLVR1 ended at a site 280 amino acids upstream of the termination codon of the bovine BLVR ORF, so the deduced mouse BLVR protein lacked the corresponding transmembrane and cytoplasmic regions of the predicted bovine BLVR protein. No significant hydrophobic region was found in the mouse protein. Recently, a human cDNA which was highly homologous (69.6% homology) to the mouse BLVR gene was reported. The cDNA encodes the delta subunit of the human adaptor-related protein complex AP-3, which aligned almost collinearly with the mouse BLVR protein. AP-3 and all other related adaptor protein complexes have been shown to be associated with intracellular vesicles but not with the cell surface. Thus, the mouse BLVR homolog appeared to be the mouse AP-3 delta subunit itself or closely related to it, but the bovine BLVR gene seemed slightly different from the adaptor subunit gene family.
克隆出了一种与牛白血病病毒受体（BLVR）基因的牛cDNA高度同源的小鼠cDNA（mBLVR1）。mBLVR1 cDNA长4730 bp，几乎覆盖了mRNA的全长（约5 kb），并包含一个编码1199个氨基酸的蛋白质的开放阅读框（ORF）。虽然牛BLVR蛋白被认为是一种I型跨膜蛋白，但mBLVR1编码的推导蛋白似乎不是典型的跨膜蛋白。mBLVR1的ORF在牛BLVR ORF终止密码子上游280个氨基酸处结束，因此推导的小鼠BLVR蛋白缺少预测的牛BLVR蛋白相应的跨膜和细胞质区域。在小鼠蛋白中未发现明显的疏水区域。最近，报道了一种与小鼠BLVR基因高度同源（同源性为69.6%）的人cDNA。该cDNA编码人衔接蛋白相关蛋白复合物AP-3的δ亚基，其与小鼠BLVR蛋白几乎呈共线性排列。AP-3和所有其他相关衔接蛋白复合物已被证明与细胞内囊泡相关，而与细胞表面无关。因此，小鼠BLVR同源物似乎是小鼠AP-3δ亚基本身或与之密切相关，但牛BLVR基因似乎与衔接子亚基基因家族略有不同。
