Fanali S, Aturki Z, Desiderio C
Istituto di Cromatografia del C.N.R., Monterolondo Scalo, Roma, Italy.
Enantiomer. 1999;4(3-4):229-41.
Capillary electrophoresis (CE) is a powerful tool for the analysis of chiral compounds of pharmaceutical interest. The separation of enantiomers can be achieved using a chiral environment responsible for the diastereoisomers formation (stable or labile in the indirect or direct separation method, respectively). A wide number of chiral selectors have been employed in CE and among them cyclodextrins or their derivatives and antibiotics are the most used stereoselective agents. The review surveys the chiral separation of drugs using the above mentioned chiral selectors by CE. The main parameters influencing the enantioresolution, e.g., chiral selector type and concentration, buffer type, concentration, pH, organic modifier as well as the capillary temperature are discussed. Finally some selected applications to real samples such as pharmaceutical formulations, serum, urine are also discussed.
毛细管电泳（CE）是分析具有药学意义的手性化合物的强大工具。对映体的分离可通过形成非对映异构体的手性环境来实现（分别在间接或直接分离方法中稳定或不稳定）。毛细管电泳中已采用了大量手性选择剂，其中环糊精及其衍生物和抗生素是最常用的立体选择性试剂。本文综述了利用上述手性选择剂通过毛细管电泳对手性药物的分离。讨论了影响对映体拆分的主要参数，如手性选择剂的类型和浓度、缓冲液类型、浓度、pH值、有机改性剂以及毛细管温度。最后还讨论了一些在实际样品（如药物制剂、血清、尿液）中的选定应用。
