Fournier R E, Pardee A B
Proc Natl Acad Sci U S A. 1975 Mar;72(3):869-73. doi: 10.1073/pnas.72.3.869.
The progress of mononucleate and cytochalasin-B-induced binucleate cells through the G1 period of the growth cycle has been studied. DNA synthesis was initiated in synchrony in both of the nuclei of cells rendered binucleate by a brief pulse of cytochalasin B. Furthermore, populations of these cells traversed the G1 period significantly faster than their mononucleate counterparts. That this effect was due to the presence of two nuclei in a common cytoplasm and not to the increased size of binucleate cells was suggested by studies of cells with altered nucleocytoplasmic ratios. No correlation was observed between protein content per cell and the rate of progress through G1. Evidence for the existence of nuclear-nuclear cooperation that results in a shortening of the G1 period is discussed.
研究了单核细胞和细胞松弛素B诱导的双核细胞在生长周期G1期的进程。通过细胞松弛素B的短暂脉冲处理使细胞形成双核后，两个细胞核中的DNA合成同步启动。此外，这些细胞群体穿越G1期的速度明显快于单核细胞。通过对核质比改变的细胞进行研究表明，这种效应是由于共同细胞质中存在两个细胞核，而不是由于双核细胞尺寸的增加。未观察到每个细胞的蛋白质含量与穿越G1期的速率之间存在相关性。文中讨论了导致G1期缩短的核-核合作存在的证据。
