Lustig S, Kosower N S, Pluznik D H, Kosower E M
Proc Natl Acad Sci U S A. 1977 Jul;74(7):2884-8. doi: 10.1073/pnas.74.7.2884.
Treatment of a line of Friend leukemia cells with a dispersion of the membrane mobility agent, A2C, yields cells that undergo successive nuclear divisions without cytokinesis, resulting eventually in cells with as many as 30 nuclei. Neither the DNA replication rate of the cells nor the generation time is different after treatment; in addition, the multiple nuclei divide synchronously, and the chromosome number corresponds to the number of nuclei in the cell. Inhibition of cytokinesis is not observed if the cells are washed with reagent-free medium within 1 hr of treatment, but is observed if washing is delayed for 24 hr. Membrane mobility agent loaded with the fluorescent probe, Flomol F20C, leads to fluorescent membrane; fluorescence disappears from the membrane after a change of medium within 1 hr, but not after a change of medium within 24 hr. Some stages in the overall development resemble those seen for cytochalasin B inhibition of cytokinesis, although the mechanisms may well be different for the inhibition promoted by membrane mobility agent. The inhibition of cytokinesis by A2C provides a potentially interesting means of studying cytokinesis and the regulation of differentiation.
用膜流动性剂A2C的分散液处理弗氏白血病细胞系，会产生细胞，这些细胞在没有胞质分裂的情况下进行连续的核分裂，最终产生多达30个核的细胞。处理后，细胞的DNA复制速率和代时均无差异；此外，多个核同步分裂，染色体数目与细胞中的核数目相对应。如果在处理后1小时内用无试剂培养基洗涤细胞，则未观察到胞质分裂受到抑制，但如果洗涤延迟24小时，则会观察到胞质分裂受到抑制。负载荧光探针Flomol F20C的膜流动性剂会导致膜发出荧光；在1小时内更换培养基后，膜上的荧光消失，但在24小时内更换培养基后则不会消失。尽管膜流动性剂促进的抑制机制可能与细胞松弛素B抑制胞质分裂的机制大不相同，但总体发育过程中的某些阶段与细胞松弛素B抑制胞质分裂时所见的阶段相似。A2C对胞质分裂的抑制为研究胞质分裂和分化调控提供了一种潜在有趣的方法。
