通过流动系统进行染色体测量和分选。

Chromosome measurement and sorting by flow systems.

作者信息

Gray J W, Carrano A V, Steinmetz L L, Van Dilla M A, Moore D H, Mayall B H, Mendelsohn M L

出版信息

Proc Natl Acad Sci U S A. 1975 Apr;72(4):1231-4. doi: 10.1073/pnas.72.4.1231.

DOI:10.1073/pnas.72.4.1231

PMID:1055399

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC432505/

Abstract

A flow microfluorometer was used to measure metaphase chromosomes in suspension at rates up to 100,000 per min. Chromosomes from cells of the Chinese hamster M3-1 cell line were isolated, stained for DNA with the fluorescent dye ethidium bromide, and analyzed for DNA content. Nine distinct peaks were resolved that correspond well with independent chromosomal DNA measurements made with a high-resolution scanning cytophotometer. Chromosomes were sorted from each peak by an electronic cell sorter. Visual examination of each fraction indicated the purity of the sorted chromosomes. This novel technology allows separation of purified populations of individual chromosomes suitable for biochemical and biological characterizations.

摘要

使用流式细胞荧光仪以每分钟高达100,000个的速率测量悬浮状态下的中期染色体。分离出中国仓鼠M3-1细胞系细胞的染色体，用荧光染料溴化乙锭对DNA进行染色，并分析DNA含量。分辨出九个不同的峰，这些峰与用高分辨率扫描细胞光度计进行的独立染色体DNA测量结果非常吻合。通过电子细胞分选仪从每个峰中分离出染色体。对每个组分的目视检查表明了分选染色体的纯度。这项新技术能够分离出适合进行生化和生物学特性分析的单个染色体的纯化群体。