通过流式细胞荧光测定法进行高速定量核型分析。

High-speed quantitative karyotyping by flow microfluorometry.

作者信息

Gray J W, Carrano A V, Moore D H, Steinmetz L L, Minkler J, Mayall B H, Mendelsohn M L, Van Dilla M A

出版信息

Clin Chem. 1975 Aug;21(9):1258-62.

Abstract

We describe a new flow-system technique for high-speed quantitative karyotyping. Metaphase chromosomes are isolated from cells, stained with a DNA-specific fluorochrome, and individually measured for stain content at rates up to 10(5)/min in a flow microfluorometer. The resulting distribution contains quantitative information about the relative frequency of occurrence and the chromosomal DNA content of the measured chromosomes. Such analyses of Chinese hamster chromosomes are in good agreement with independent results from scanning cytophotometry and with cytogenetic observations of the banded chromosomes. Analyses of two karyotypically different clones of Chinese hamseter M3-1 lines provide the quantitative characterization of two stable translocations. Initial data on human chromosomes are presented and agree well with results of scanning cytophotometry and suggest potential clinical applications as preparative and instrumental techniques improve.

摘要

我们描述了一种用于高速定量核型分析的新流动系统技术。从中期染色体从细胞中分离出来，用一种DNA特异性荧光染料染色，并在流动微荧光计中以高达10(5)/分钟的速率对每条染色体的染色含量进行单独测量。所得分布包含有关所测染色体出现的相对频率和染色体DNA含量的定量信息。对中国仓鼠染色体的此类分析与扫描细胞光度法的独立结果以及带型染色体的细胞遗传学观察结果高度一致。对中国仓鼠M3-1系两个核型不同的克隆的分析提供了两种稳定易位的定量特征。还展示了关于人类染色体的初步数据，这些数据与扫描细胞光度法的结果高度一致，并表明随着制备和仪器技术的改进，具有潜在的临床应用价值。