用于中期染色体流式细胞术的脱氧核糖核酸染色剂选择策略。

Strategies for choosing a deoxyribonucleic acid stain for flow cytometry of metaphase chromosomes.

作者信息

Jensen R H, Langlois R G, Mayall B H

出版信息

J Histochem Cytochem. 1977 Aug;25(8):954-64. doi: 10.1177/25.8.70463.

DOI:10.1177/25.8.70463

Abstract

Requirements for flow cytometry of metaphase chromosomes stained with three deoxyribonucleic acid (DNA)-specific fluorescent dyes--Hoechst 33258, Chromomycin A3, and ethidium bromide--are reviewed. Fluorescence properties of these three stains when bound to mitotic cells or to chromosomes in suspension are measured and compared with fluorescence properties when bound to DNA in solution. Conditions are given for high resolution flow cytometry of Chinese hamster chromosomes stained with each of the fluorophors, and histograms are presented that exhibit differences in relative peak position and area. Energy transfer fluorescence between two DNA stains is presented as a potentially useful new parameter for flow cytometry of chromosomes and is illustrated by fluorescence energy transfer from Chromomycin A3 to ethidium bromide when simultaneously bound to hamster mitotic cells.

摘要

本文综述了使用三种脱氧核糖核酸（DNA）特异性荧光染料——Hoechst 33258、放线菌素A3和溴化乙锭——对中期染色体进行流式细胞术分析的要求。测定了这三种染料与有丝分裂细胞或悬浮染色体结合时的荧光特性，并与它们与溶液中的DNA结合时的荧光特性进行了比较。给出了用每种荧光染料染色的中国仓鼠染色体进行高分辨率流式细胞术分析的条件，并展示了直方图，这些直方图显示了相对峰位置和面积的差异。两种DNA染料之间的能量转移荧光被提出作为染色体流式细胞术一个潜在有用的新参数，并通过同时与仓鼠有丝分裂细胞结合时从放线菌素A3到溴化乙锭的荧光能量转移进行了说明。