Schleicher U M, Lopez Cotarelo C, Andreopoulos D, Handt S, Ammon J
Klinik für Strahlentherapie, Universitätsklinikum der Rheinisch-Westfälischen Technischen Hochschule Aachen.
Med Klin (Munich). 1999 Oct 15;94 Suppl 3:35-8. doi: 10.1007/BF03042188.
Sodium selenite is applied in tumor patients during chemo- or radiotherapy due to its cytoprotective effects. Aim of our study was to evaluate the effect of exposure with sodium selenite on proliferation of human endothelial and tumor cells after irradiation.
We studied the proliferative activity of human umbilical vein endothelial cells in comparison to tumor cells of the HeLa, MIA Paca-2 and SiHa cell line after single-dose irradiation with 2 or 10 Gy and controls without irradiation. All cells had been exposed to different concentrations of sodium selenite prior to irradiation. Evaluation was done by BrdU-ELISA.
Exposure of human endothelial cells with sodium selenite concentrations > or = 100 micrograms/l led to an increase of BrdU proliferation index. This effect was markedly weaker in HeLa cells and not found in SiHa and MIA Paca-2.
High concentrations of sodium selenite can counteract the decrease of proliferative activity caused by irradiation in human endothelial cells and thus exert a radioprotective effect on these cells. This effect was observed by far stronger in endothelial cells than in tumor cells, implying the possible clinical use of sodium selenite as a protective agent for normal tissue in radiotherapy.
由于亚硒酸钠具有细胞保护作用，因此在肿瘤患者化疗或放疗期间应用亚硒酸钠。本研究的目的是评估照射后亚硒酸钠暴露对人内皮细胞和肿瘤细胞增殖的影响。
我们研究了人脐静脉内皮细胞与HeLa、MIA Paca - 2和SiHa细胞系的肿瘤细胞在单次2或10 Gy照射后以及未照射对照下的增殖活性。所有细胞在照射前均暴露于不同浓度的亚硒酸钠。通过BrdU - ELISA进行评估。
暴露于浓度≥100微克/升的亚硒酸钠使人内皮细胞的BrdU增殖指数增加。这种作用在HeLa细胞中明显较弱，而在SiHa和MIA Paca - 2细胞中未发现。
高浓度的亚硒酸钠可抵消照射引起的人内皮细胞增殖活性降低，从而对这些细胞发挥辐射防护作用。在内皮细胞中观察到的这种作用远比在肿瘤细胞中强得多，这意味着亚硒酸钠可能作为放疗中正常组织的保护剂具有临床应用价值。
