Nucleic acid immunization: concepts and techniques associated with third generation vaccines.

作者信息

Hasan U A, Abai A M, Harper D R, Wren B W, Morrow W J

机构信息

Molecular Immunology and Infection Research Group, Department of Immunology, St. Bartholomew's and the Royal London School of Medicine and Dentistry, 38 Little Britain, London, UK.

出版信息

J Immunol Methods. 1999 Oct 29;229(1-2):1-22. doi: 10.1016/s0022-1759(99)00104-0.

DOI:10.1016/s0022-1759(99)00104-0

PMID:10556687

Abstract

A radical change in vaccine methodology arrived nine years ago with the advent of nucleic acid immunization. Aspects such as plasmid design, gene selection, the use of immunostimulatory complexes and clinical trials are discussed in this review. Furthermore, concepts and protocols involved in the construction, evaluation and immunization of a DNA vaccine have been examined as new strategies to enhance this technology continues to grow.

摘要