Yan S M, Finato N, Di Loreto C, Beltrami C A
Department of Pathology, University of Udine, Italy.
Anal Quant Cytol Histol. 1999 Apr;21(2):174-80.
To determine the alteration of nuclear size in myocardial cells and the relationship between nuclear size and DNA ploidy classes in normal and cardiomyopathic human hearts.
The study group consisted of 46 hearts obtained at biopsy. These patients had undergone cardiac transplantation for intractable congestive heart failure (18 cases with ischemic cardiomyopathy and 28 cases with idiopathic dilated cardiomyopathy). Another 10 hearts were collected at autopsy and used as control hearts according to preautopsy, autopsy and histology criteria. One hundred fibroblasts and 200 myocytes were evaluated in each ventricle. The nuclear area and DNA content were estimated using image cytometry.
End-stage ischemic and dilated cardiomyopathies were characterized by an increase in nuclear size of both the myocyte and nonmyocyte population. The nuclear area of interstitial cells increased about 30% in cardiomyopathic hearts. Augmentation of average nuclear area of myocytes was 1.2-fold in the ischemic group and about 1.5-fold in the dilated group as compared with the control group. Also, a tendency was found for the coefficient of variation of average nuclear area to decrease in the interstitial cell population and increased in the myocyte population in cardiomyopathic situations. Furthermore, the nuclear area of myocytes enlarged as augmentation of nuclear DNA content. The relative nuclear areas of myocytes can be presented as: 2c:4c:8c:16c :32c:64c = 1:1.65:2.75:4.60:7.25:9.18.
The increase in nuclear size follows either one of two different processes: the first does not involve an increase in DNA content, whereas the second is concomitant with an incremental increase in DNA content. In the first instance, the enlargement of nuclear size is limited. In the second, augmentation of nuclear size can become very impressive. In end-stage ischemic and dilated cardiomyopathies, the nuclear growth of myocytes and interstitial cells may be due to different mechanisms. Enlargement of the nuclear area of myocytes represents a complex process, including simple nuclear hypertrophy, polyploidization and multinucleation. The main pattern of nuclear growth of interstitial cells is nuclear hypertrophy without an increase in DNA content.
确定正常和心肌病患者心脏中心肌细胞核大小的改变以及核大小与DNA倍体类别的关系。
研究组由46例活检获取的心脏组成。这些患者因难治性充血性心力衰竭接受心脏移植（18例缺血性心肌病和28例特发性扩张型心肌病）。根据尸检前、尸检和组织学标准，另外收集10例心脏作为对照心脏。在每个心室中评估100个成纤维细胞和200个心肌细胞。使用图像细胞术估计核面积和DNA含量。
终末期缺血性和扩张型心肌病的特征是心肌细胞和非心肌细胞群体的核大小增加。心肌病心脏中间质细胞的核面积增加约30%。与对照组相比，缺血组心肌细胞平均核面积增加1.2倍，扩张组约增加1.5倍。此外，发现在心肌病情况下，间质细胞群体平均核面积变异系数有下降趋势，而心肌细胞群体有增加趋势。此外，随着核DNA含量的增加，心肌细胞核面积增大。心肌细胞的相对核面积可表示为：2c:4c:8c:16c:32c:64c = 1:1.65:2.75:4.60:7.25:9.18。
核大小的增加遵循两种不同过程之一：第一种不涉及DNA含量增加，而第二种与DNA含量的逐渐增加同时发生。在第一种情况下，核大小的增大是有限的。在第二种情况下，核大小的增加可能会非常显著。在终末期缺血性和扩张型心肌病中，心肌细胞和间质细胞的核生长可能是由于不同机制。心肌细胞核面积的增大代表一个复杂过程，包括单纯核肥大、多倍体化和多核化。间质细胞核生长的主要模式是核肥大而DNA含量不增加。
