Asfour B, Hare J M, Kohl T, Baba H A, Kass D A, Chen K, Tjan T D, Hammel D, Weyand M, Hruban R H, Scheld H H, Byrne B J
Department of Thoracic and Cardiovascular Surgery, Westfalian Wilhelms University, Münster, Germany.
J Heart Lung Transplant. 1999 Oct;18(10):927-36. doi: 10.1016/s1053-2498(99)00062-5.
The widely used non-volume-loaded abdominal heterotopic heart transplant (NL) in rats undergoes atrophy after transplantation. Various techniques have been designed to load the transplanted heart because of its potential immunological impact. Our aim was to create a volume-loaded heterotopic heart transplantation model (VL) capable of ejection and practical for routine studies. Using this model, we tested the hypothesis that VL isografts would retain myocardial performance comparable to native hearts (NH).
Heterotopic hearts were transplanted using and end-to-side anastomosis between the donor's superior vena cava and the recipient's abdominal inferior vena cava. The right ventricle loads the left ventricle (LV) via a direct anastomosis of the pulmonary artery to the left atrium. The LV ejects volume through an end-to-side anastomosis of the donor's aorta to the recipient's abdominal aorta. Hemodynamic data (systolic and diastolic LV pressures, dP/dt max and min, tau) were studied in-situ (at baseline and after adding volume) and in a Langendorff perfusion system (at baseline and after stimulation with isoproterenol) 2 weeks after transplantation.
In situ systolic pressure and diastolic function of VL was superior to NL, and beta-adrenergic stimulated performance in the Langendorff perfusion of VL showed hemodynamic performance equivalent to NH, unlike NL which had a diminished response.
This technique results in a volume-loaded ejecting heart transplant model that preserves anatomical structures. The VL can be evaluated in situ and after explantation in Langendorff perfusion system and may offer advantages if workload of the graft is of significance to the study performed.
大鼠广泛应用的非容量负荷性腹部异位心脏移植（NL）在移植后会发生萎缩。由于其潜在的免疫影响，已设计了各种技术来对移植心脏进行负荷。我们的目的是创建一种能够射血且适用于常规研究的容量负荷性异位心脏移植模型（VL）。使用该模型，我们检验了以下假设：VL同基因移植心脏将保留与正常心脏（NH）相当的心肌功能。
采用供体上腔静脉与受体腹部下腔静脉端侧吻合进行异位心脏移植。右心室通过肺动脉与左心房直接吻合来负荷左心室（LV）。左心室通过供体主动脉与受体腹部主动脉端侧吻合射血。在移植后2周，在原位（基线和增加容量后）以及Langendorff灌注系统中（基线和用异丙肾上腺素刺激后）研究血流动力学数据（左心室收缩压和舒张压、dP/dt最大值和最小值、弛豫时间常数）。
VL的原位收缩压和舒张功能优于NL，并且在Langendorff灌注中，VL的β-肾上腺素能刺激后的功能显示出血流动力学性能与NH相当，而NL的反应减弱。
该技术产生了一种保留解剖结构的容量负荷性射血心脏移植模型。VL可以在原位以及在Langendorff灌注系统中取出后进行评估，如果移植物的负荷对所进行的研究具有重要意义，可能会具有优势。
