Ohmi M, Yokoyama H, Nakame T, Murata S, Akimoto H, Tabayashi K, Mohri H
Department of Thoracic and Cardiovascular Surgery, Tohoku University School of Medicine, Sendai, Japan.
J Heart Lung Transplant. 1992 Nov-Dec;11(6):1147-50.
To study hemodynamics together with various aspects of rejection after experimental heart transplantation, we developed a technique to produce a working left heart model of heterotopic (abdominal) heart transplantation. The interatrial septum and tricuspid valve of the donor heart are removed. The pulmonary arterial trunk, pulmonary veins, and inferior vena cava are ligated, and the stumps of the donor aorta and superior vena cava are anastomosed in an end-to-side fashion to the recipient abdominal aorta and inferior vena cava, respectively. Arterial blood from the recipient abdominal aorta thus perfuses the donor myocardium through the coronary artery, and the donor left ventricle receives venous blood from the recipient inferior vena cava as preload. In this model, the donor left ventricle does not pump out enough venous blood to desaturate the recipient femoral arterial blood but does generate approximately the same pressure as the recipient's heart. This model is reproducible, easy to manage, and can be applied to heterotopic heart transplantation in various experimental animals including rats.
为了研究实验性心脏移植后的血流动力学以及排斥反应的各个方面，我们开发了一种技术，以构建一个异位（腹部）心脏移植的左心工作模型。移除供体心脏的房间隔和三尖瓣。结扎肺动脉干、肺静脉和下腔静脉，将供体主动脉和上腔静脉的残端分别以端侧吻合的方式与受体腹主动脉和下腔静脉吻合。来自受体腹主动脉的动脉血通过冠状动脉灌注供体心肌，供体左心室接受来自受体下腔静脉的静脉血作为前负荷。在这个模型中，供体左心室泵出的静脉血不足以使受体股动脉血饱和度降低，但能产生与受体心脏大致相同的压力。这个模型可重复、易于管理，并且可应用于包括大鼠在内的各种实验动物的异位心脏移植。
