[1994年至1996年某大学医院大肠杆菌中广谱β-内酰胺酶的发生率]
[Incidence of extended spectrum beta lactamase in Escherichia coli in a university hospital from 1994-1996].
作者信息
Sabaté M, Vergés C, Miró E, Mirelis B, Navarro F, del Río E, Prats G
机构信息
Servicio de Microbiología, Hospital de la Santa Creu i Sant Pau, Barcelona.
出版信息
Enferm Infecc Microbiol Clin. 1999 Oct;17(8):401-4.
BACKGROUND
The aim of this study was to evaluate the incidence of TEM- and SHV-type extended spectrum betalactamases (ESBLs) in Escherichia coli in a 700-bed teaching hospital between 1994 and 1996.
MATERIAL AND METHODS
Strains that presented reduced diameters to third-generation cephalosporins, as identified by disc diffusion techniques, were studied. The betalactamases involved were characterized by determination of the isoelectric point, hydrolysis profile, gene detection by polymerase chain reaction (PCR), and sequencing of the amplified products.
RESULTS
96 strains (1.4%) out of 7,054 strains of E. coli isolated between 1994 and 1996 showed decreased susceptibility to third-generation cephalosporins and only 4 strains (0.06%) produced ESBLs. Two strains produced SHV-2 and two produced TEM-12.
CONCLUSIONS
The contribution of ESBL production to resistance to third-generation cephalosporins was low: 0.06% of all the E. coli strains isolated between 1994 and 1996.
背景
本研究旨在评估1994年至1996年期间，一家拥有700张床位的教学医院中大肠杆菌中TEM型和SHV型超广谱β-内酰胺酶（ESBLs）的发生率。
材料与方法
对通过纸片扩散法鉴定出对第三代头孢菌素抑菌圈直径减小的菌株进行研究。通过测定等电点、水解谱、聚合酶链反应（PCR）基因检测以及扩增产物测序来鉴定相关的β-内酰胺酶。
结果
1994年至1996年间分离出的7054株大肠杆菌中，96株（1.4%）对第三代头孢菌素的敏感性降低，只有4株（0.06%）产生ESBLs。两株产生SHV-2，两株产生TEM-12。
结论
ESBLs产生对第三代头孢菌素耐药性的影响较低：在1994年至1996年间分离出的所有大肠杆菌菌株中占0.06%。
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