Wolf C, Rentsch J, Hübner P
Laboratory of Food Chemistry and Department of Chemistry and Biochemistry, University of Berne, Switzerland.
J Agric Food Chem. 1999 Apr;47(4):1350-5. doi: 10.1021/jf9808426.
A method for identification of game species has been developed on the basis of the amplification of a specific part of the mitochondrial genome (tRNA(Glu)/cytochrome b) using the polymerase chain reaction (PCR). To distinguish between several game species, the obtained 464-bp-long PCR products were cut with different restriction endonucleases (RE) resulting in species-specific restriction fragment length polymorphism (RFLP). Even closely related deer species could be distinguished by application of one or two RE. Natural polymorphisms of the target sequence within one species were examined for red deer (Cervus elaphus), and base pair substitutions were identified affecting the RFLP pattern.
基于聚合酶链反应（PCR）对线粒体基因组特定部分（tRNA(Glu)/细胞色素b）进行扩增，已开发出一种鉴定狩猎动物物种的方法。为区分几种狩猎动物物种，将获得的464bp长的PCR产物用不同的限制性内切酶（RE）切割，从而产生物种特异性的限制性片段长度多态性（RFLP）。通过应用一种或两种RE，甚至亲缘关系密切的鹿种也能被区分开来。对马鹿（Cervus elaphus）一个物种内目标序列的自然多态性进行了研究，并鉴定出影响RFLP模式的碱基对替换。
