Fajardo Violeta, González Isabel, López-Calleja Inés, Martín Irene, Hernández Pablo E, García Teresa, Martín Rosario
Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain.
J Agric Food Chem. 2006 Feb 22;54(4):1144-50. doi: 10.1021/jf051766r.
PCR-RFLP analysis has been applied to the identification of meats from red deer (Cervus elaphus), fallow deer (Dama dama), roe deer (Capreolus capreolus), cattle (Bos taurus), sheep (Ovis aries), and goat (Capra hircus). PCR amplification was carried out using a set of primers flanking a conserved region of approximately 712 base pairs from the mitochondrial 12S rRNA gene. Restriction site analysis based on sequence data from this DNA fragment permitted the selection of MseI, MboII, BslI, and ApoI endonucleases for species identification. The restriction profiles obtained when amplicons were digested with the chosen enzymes allowed the unequivocal identification of all domestic and game meat species analyzed in the present work.
聚合酶链反应-限制性片段长度多态性分析（PCR-RFLP）已应用于马鹿（Cervus elaphus）、黇鹿（Dama dama）、狍（Capreolus capreolus）、牛（Bos taurus）、绵羊（Ovis aries）和山羊（Capra hircus）肉类的鉴定。使用一组引物对线粒体12S rRNA基因中约712个碱基对的保守区域进行PCR扩增。基于该DNA片段的序列数据进行的限制性位点分析，选择了MseI、MboII、BslI和ApoI核酸内切酶用于物种鉴定。用所选酶消化扩增子后获得的限制性图谱，能够明确鉴定本研究中分析的所有家养和野味肉类物种。
