Calhoun N R, Smith J C, Becker K L
Oral Surg Oral Med Oral Pathol. 1975 May;39(5):698-706. doi: 10.1016/0030-4220(75)90029-8.
The effect of dietary zinc deficiency was studies in ectopic bone formation subsequent to Achilles tenotomy and also following the implantation of demineralized bone matrix in the muscle of rats. Three experiments were performed. The first was designed to investigate the relationship between zinc and calcium concentration during the formation of ectopic bone in rats fed a commercial laboratory ration, the second concerned the effects of dietary zinc deficiency on ectopic bone formation, and the third studies the subsequent effects of dietary zinc repletion on ectopic bone formation. The results indicated that, with the commercial ration, zinc increased concomitantly with calcium during ectopic bone formation in rats. Dietary zinc deficiency caused a retardation of ectopic bone formation and a significant reduction of in situ zinc and calcium concentration. Dietary zinc repletion to zinc-deficient animals restored the zinc concentration in ectopic bone to a level comparable to that of zinc-sufficient animals. Thus, these experiments present strong evidence that zinc plays an active role in bone metabolism.
研究了膳食锌缺乏对大鼠跟腱切断术后异位骨形成以及脱矿骨基质植入肌肉后异位骨形成的影响。进行了三项实验。第一项实验旨在研究喂食商业实验室饲料的大鼠异位骨形成过程中锌与钙浓度之间的关系；第二项实验关注膳食锌缺乏对异位骨形成的影响；第三项实验研究膳食补充锌对异位骨形成的后续影响。结果表明，在喂食商业饲料的情况下，大鼠异位骨形成过程中锌与钙同时增加。膳食锌缺乏导致异位骨形成迟缓，原位锌和钙浓度显著降低。给缺锌动物补充膳食锌可使异位骨中的锌浓度恢复到与锌充足动物相当的水平。因此，这些实验提供了有力证据，证明锌在骨代谢中起积极作用。
