人类RNA聚合酶II亚基全组分之间的相互作用。

Interactions between the full complement of human RNA polymerase II subunits.

作者信息

Schaller S, Grandemange S, Shpakovski G V, Golemis E A, Kedinger C, Vigneron M

机构信息

Institut de Génétique et de Biologie Moléculaire et Cellulaire (CNRS/INSERM/ULP), BP 163, 67404, Illkirch, France.

出版信息

FEBS Lett. 1999 Nov 19;461(3):253-7. doi: 10.1016/s0014-5793(99)01441-6.

DOI:10.1016/s0014-5793(99)01441-6

PMID:10567706

Abstract

As an approach to elucidating the rules governing the assembly of human RNA polymerase II (hRPB), interactions between its subunits have been systematically analyzed. Eleven of the 12 expected hRPB subunits have previously been tested for reciprocal interactions (J. Biol. Chem. 272 (1997) 16815-16821). We now report the results obtained for the last subunit (hRPB4; Mol. Cell. Biol. 18 (1998) 1935-1945) and propose an essentially complete picture of the potential interactions occurring within hRPB. Finally, complementation experiments in yeast indicated that hRPB4 expression efficiently cured both heat and cold-sensitivity of RPB4-lacking strains, supporting the existence of conserved functional subunit interactions.

摘要

作为阐明人类RNA聚合酶II（hRPB）组装规则的一种方法，对其亚基之间的相互作用进行了系统分析。之前已对12个预期的hRPB亚基中的11个进行了相互作用测试（《生物化学杂志》272卷（1997年）16815 - 16821页）。我们现在报告最后一个亚基（hRPB4；《分子与细胞生物学》18卷（1998年）1935 - 1945页）的研究结果，并提出hRPB内潜在相互作用的基本完整图景。最后，酵母中的互补实验表明，hRPB4的表达有效治愈了缺乏RPB4菌株的热敏感性和冷敏感性，支持了保守的功能性亚基相互作用的存在。