Hart P H, Grimbaldeston M A, Swift G J, Hosszu E K, Finlay-Jones J J
Department of Microbiology & Infectious Diseases, School of Medicine, Flinders University, Adelaide, Australia.
Photochem Photobiol. 1999 Nov;70(5):807-12.
Many studies have implicated cis-urocanic acid (cis-UCA) in UVB-induced immunomodulation. The strongest evidence came from studies in mice whereby a cis-UCA antibody blocked UVB-induced suppression of delayed-type hypersensitivity responses. Furthermore, in several studies, the cis-UCA antibody at least partially reversed UVB suppression of contact hypersensitivity responses. Previous reports suggested that cis-UCA was immunomodulatory through its effects on keratinocytes, Langerhans cells, fibroblasts, T lymphocytes, natural killer cells and monocytes/macrophages. As dermal mast cells were recently demonstrated to be critical to UVB-induced systemic suppression of certain delayed-type and contact hypersensitivity responses, we investigated whether they were involved in the processes by which cis-UCA was immunomodulatory. Not only was there a correlation between dermal mast cell prevalence and the degree of susceptibility of different strains of mice to the immunomodulatory effects of cis-UCA, there was also a functional link. Mast cell-depleted Wf/Wf mice were rendered susceptible to immunomodulation by cis-UCA injected subcutaneously only after their dorsal skin had been reconstituted with bone marrow-derived mast cell precursors. These studies suggest that mast cells are critical to the processes by which cis-UCA suppresses systemic contact hypersensitivity responses to the hapten, trinitrochlorobenzene, in mice.
许多研究表明顺式尿刊酸（cis-UCA）参与紫外线B（UVB）诱导的免疫调节。最有力的证据来自对小鼠的研究，在这些研究中，一种顺式尿刊酸抗体可阻断UVB诱导的迟发型超敏反应抑制。此外，在多项研究中，顺式尿刊酸抗体至少部分逆转了UVB对接触性超敏反应的抑制。先前的报告表明，顺式尿刊酸通过其对角质形成细胞、朗格汉斯细胞、成纤维细胞、T淋巴细胞、自然杀伤细胞和单核细胞/巨噬细胞的作用而具有免疫调节作用。由于最近证明真皮肥大细胞对UVB诱导的某些迟发型和接触性超敏反应的全身抑制至关重要，我们研究了它们是否参与顺式尿刊酸发挥免疫调节作用的过程。不仅真皮肥大细胞的患病率与不同品系小鼠对顺式尿刊酸免疫调节作用的敏感程度之间存在相关性，而且还存在功能联系。肥大细胞缺陷的Wf/Wf小鼠只有在其背部皮肤用骨髓来源的肥大细胞前体重建后，才会对皮下注射的顺式尿刊酸的免疫调节作用敏感。这些研究表明，肥大细胞对顺式尿刊酸抑制小鼠对半抗原三硝基氯苯的全身接触性超敏反应的过程至关重要。
