Calder M D, Salfen B E, Bao B, Youngquist R S, Garverick H A
Department of Animal Sciences, University of Missouri, Columbia 65211, USA.
J Anim Sci. 1999 Nov;77(11):3037-42. doi: 10.2527/1999.77113037x.
Cows with ovarian follicular cysts were treated with progesterone to determine whether a reduction in LH concentrations and initiation of ovulatory follicular waves would occur. Cysts were diagnosed using transrectal ultrasonography when single follicular structures > 20 mm or multiple structures > 15 mm in diameter were present for 7 d in the presence of low progesterone concentrations. Three groups were studied: 1) cows with normal estrous cycles (CYC, n = 8); 2) cows with untreated cysts (CYST, n = 7); and 3) cows with cysts treated with two progesterone-releasing intravaginal devices (PRID, n = 8) for 9 d. Ovaries were examined with transrectal ultrasonography, and blood samples were collected daily for analysis of progesterone and FSH. Serial blood samples for determination of mean LH and LH pulse frequency were collected on d 0 (CYST and PRID cows only), 1, 5, 9, and 10. Progesterone concentrations were higher in PRID cows than in CYST cows throughout the PRID treatment period (P < .002). On d 0, LH pulse frequency was similar (P = .10) in PRID (6.6+/-.6 pulses/8 h) and CYST cows (5.1+/-.6 pulses/8 h), but mean LH tended to be higher (P = .054) on d 0 in PRID cows (2.5+/-.2 ng/mL) than in CYST cows (1.9+/-.2 ng/mL). Mean LH and LH pulse frequency decreased (P < .002) by d 1 in PRID cows (1.1+/-.2 ng/mL, 1.8+/-.6 pulses/8 h) compared with CYST cows (2.1+/-.2 ng/mL, 5.6+/-.6 pulses/8 h) and remained lower throughout most of the experimental period. The FSH concentrations were higher (P < .01) in PRID cows than in CYC and CYST cows on d 3 and 4. The increase in FSH concentrations preceded emergence of the PRID-induced follicular wave. All PRID cows and four of seven CYST cows initiated new follicular waves during the period of PRID treatment. Follicular waves were initiated later (P < .05) in CYST cows (d 5.2+/-1.7) and PRID cows (d 5.5+/-.6) than in CYC cows (d 1.8+/-.3). Cysts were smaller (P < .01) at the end of the treatment period in PRID cows compared with CYST cows. No CYST cows ovulated, but all PRID cows ovulated newly developed follicles 3 or 4 d after PRID removal. Treatment with exogenous progesterone reduced LH in cows with cysts, and this was followed by development of normal ovulatory follicles.
用孕酮治疗患有卵巢卵泡囊肿的奶牛，以确定促黄体生成素（LH）浓度是否会降低以及排卵性卵泡波是否会启动。当在低孕酮浓度情况下，单个卵泡结构直径>20毫米或多个结构直径>15毫米持续存在7天时，通过经直肠超声检查诊断囊肿。研究了三组：1）发情周期正常的奶牛（CYC，n = 8）；2）未治疗囊肿的奶牛（CYST，n = 7）；3）用两个释放孕酮的阴道装置（PRID）治疗囊肿9天的奶牛（n = 8）。通过经直肠超声检查卵巢，并每天采集血样分析孕酮和促卵泡素（FSH）。在第0天（仅CYST和PRID组奶牛）、第1天、第5天、第9天和第10天采集连续血样以测定平均LH和LH脉冲频率。在整个PRID治疗期间，PRID组奶牛的孕酮浓度高于CYST组奶牛（P <.002）。在第0天，PRID组奶牛（6.6±0.6次脉冲/8小时）和CYST组奶牛（5.1±0.6次脉冲/8小时）的LH脉冲频率相似（P = 0.10），但PRID组奶牛在第0天的平均LH趋于更高（P = 0.054）（2.5±0.2纳克/毫升），高于CYST组奶牛（1.9±0.2纳克/毫升）。与CYST组奶牛（2.1±0.2纳克/毫升，5.6±0.6次脉冲/8小时）相比，PRID组奶牛在第1天平均LH和LH脉冲频率降低（P <.002）（1.1±0.2纳克/毫升，1.8±0.6次脉冲/8小时），并且在实验期的大部分时间内保持较低水平。在第3天和第4天，PRID组奶牛的FSH浓度高于CYC组和CYST组奶牛（P <.01）。FSH浓度的升高先于PRID诱导的卵泡波出现。所有PRID组奶牛和7头CYST组奶牛中的4头在PRID治疗期间启动了新的卵泡波。CYST组奶牛（第5.2±1.7天）和PRID组奶牛（第5.5±0.6天）启动卵泡波的时间比CYC组奶牛（第1.8±0.3天）晚（P <.05）。与CYST组奶牛相比，PRID组奶牛在治疗期结束时囊肿较小（P <.01）。没有CYST组奶牛排卵，但所有PRID组奶牛在取出PRID后3或4天排卵新发育的卵泡。用外源性孕酮治疗可降低患有囊肿奶牛的LH，随后发育出正常的排卵卵泡。
