Xiao X Q, Yang J W, Tang X C
State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, People's Republic of China.
Neurosci Lett. 1999 Nov 12;275(2):73-6. doi: 10.1016/s0304-3940(99)00695-3.
The effects of Huperzine A (HupA), a novel acetylcholinesterase inhibitor, on hydrogen peroxide (H2O2) induced cell lesion, level of lipid peroxidation and antioxidant enzyme activities were investigated in rat pheochromocytoma line PC12. Following a 6-h exposure of the cells to H2O2 (200 microM), a marked reduction in cell survival and activities of glutathione peroxidase and catalase, as well as increased production of malondialdehyde (MDA) were observed. Pretreatment of the cells with HupA (0.1-10.0 microM) prior to H2O2 exposure significantly elevated the cell survival and antioxidant enzyme activities and decreased the level of MDA. Our results indicated that in addition to its anticholinesterase effects, HupA had protective effects against free radical-induced cell toxicity, which might be beneficial for the treatment of Alzheimer's disease.
石杉碱甲（HupA）是一种新型乙酰胆碱酯酶抑制剂，本研究在大鼠嗜铬细胞瘤细胞系PC12中，考察了其对过氧化氢（H2O2）诱导的细胞损伤、脂质过氧化水平及抗氧化酶活性的影响。细胞经H2O2（200 microM）处理6小时后，细胞存活率、谷胱甘肽过氧化物酶和过氧化氢酶活性显著降低，丙二醛（MDA）生成增加。H2O2处理前用HupA（0.1 - 10.0 microM）预处理细胞，可显著提高细胞存活率和抗氧化酶活性，并降低MDA水平。我们的结果表明，除了其抗胆碱酯酶作用外，石杉碱甲对自由基诱导的细胞毒性具有保护作用，这可能对阿尔茨海默病的治疗有益。
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