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韩国传统配方药草芍药延年美白子仁丸(PJBH)对过氧化氢诱导的PC12细胞损伤的减轻作用

Attenuating effect of a traditional korean formulation, Paeng-Jo-Yeon-Nyeon-Baek-Ja-In-Hwan (PJBH), on hydrogen peroxide-induced injury in PC12 cells.

作者信息

Koo Byung-Soo, Kim Yeon-Kye, Park Kyung-Su, Chung Kang-Hyun, Kim Cheorl-Ho

机构信息

National Research Laboratory for Glycobiology, Ministry of Science and Technology of Korea, Kyungju, Kyungbuk, Korea.

出版信息

Phytother Res. 2004 Jun;18(6):488-93. doi: 10.1002/ptr.1457.

Abstract

The Paeng-Jo-Yeon-Nyeon-Baek-Ja-In-Hwan (PJBH) prescription is a dried decoctum consisting of a mixture of 18 medicinal herbs that include Semen Biotae, Fructus Torilis seu cnidii, Fructus Rubi, Herba Dendrobii, Radix Morindae officinalis, Cortex Eucommiae, Radix Aspragi, Radix Polygalae, Radix Dipsaci, Ramulus Cinnamomi, Rhizoma Acori graminei, Rhizoma Alismatis, Rhizoma Dioscoreae, Radix Ginseng, Radix Rehmanniae preparata, Fructus corni, Fructus Schisandrae and Herba Cistanches. The effect of PJBH extracts on H2O2-induced toxicity in the rat pheochromocytoma line PC12 was examined by measurements of cell lesion, level of lipid peroxidation and antioxidant enzyme activities, since free radicals are involved in neurodegeneration in Alzheimer's disease (AD). After a 30 min exposure of the cells to H2O2 (150 microM), a marked decrease in cell survival, activities of glutathione peroxidase and catalase as well as an increased production of malondialdehyde (MDA) were found. Pretreatment of the cells with PJBH (0.5-10 microg/ml) prior to H2O2 exposure significantly elevated cell survival, antioxidant enzyme activities and resulted in a decrease in the level of MDA. The effects of the PJBH on hydrogen peroxide-induced injury in PC12 cells were also examined. PJBH had a remarkable elevating effect on catalase and GSH-Px activities as well as cell survival, suggesting that cytoprotective effects of the PJBH are involved in stimulation against intermediate concentrations of H2O2-induced PC12 cell injury. The above-mentioned neuroprotective effects were also compared with the effect of tacrine. The results suggest that PJBH has potential for use as a novel neuronal therapeutic agent.

摘要

平调养颜更年白甲丸(PJBH)方剂是一种由18味草药混合而成的干煎剂,这些草药包括柏子仁、白蒺藜、覆盆子、石斛、巴戟天、杜仲、天门冬、远志、续断、桂枝、石菖蒲、泽泻、山药、人参、熟地黄、山茱萸、五味子和肉苁蓉。由于自由基参与阿尔茨海默病(AD)的神经退行性变,因此通过测量细胞损伤、脂质过氧化水平和抗氧化酶活性,研究了PJBH提取物对过氧化氢诱导的大鼠嗜铬细胞瘤PC12细胞毒性的影响。在细胞暴露于过氧化氢(150 microM)30分钟后,发现细胞存活率、谷胱甘肽过氧化物酶和过氧化氢酶活性显著降低,丙二醛(MDA)产量增加。在过氧化氢暴露前用PJBH(0.5 - 10微克/毫升)预处理细胞,可显著提高细胞存活率、抗氧化酶活性,并导致MDA水平降低。还研究了PJBH对过氧化氢诱导的PC12细胞损伤的影响。PJBH对过氧化氢酶和谷胱甘肽过氧化物酶活性以及细胞存活率有显著的提高作用,表明PJBH的细胞保护作用涉及对中等浓度过氧化氢诱导的PC12细胞损伤的刺激。还将上述神经保护作用与他克林的作用进行了比较。结果表明,PJBH有潜力作为一种新型的神经元治疗剂。

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