Moschettini D, Galieni P, Valensin P E, Laszlo D, Scalia G, Tozzi M, Lauria F, Donati D
Dipartimento di Biologia Molecolare, Sezione di Microbiologia, Università di Siena, Siena, Italy.
J Med Virol. 2000 Jan;60(1):39-42.
After primary infection in early life, human herpesvirus 6 (HHV-6) remains latent in the body and may reactivate in subjects with poor immune status. A 180-day longitudinal study of HHV-6 infection was carried out in 23 autologous bone marrow transplant recipients to evaluate reactivation of HHV-6; two of these patients underwent a double transplant. The patients were monitored prospectively for HHV-6 DNA in peripheral blood mononuclear cells (PBMC) by hot start nested PCR. Positive samples were typed by the enzymatic restriction protocol. Positive plasma samples were also tested for HHV-6 DNA. Antibodies against HHV-6 were measured by immunofluorescence. Five and two out of 23 patients had intermittent and persistent positivity to HHV-6 DNA in PBMCs, respectively; four patients carried variant B, and the other three patients both A and B. None of the respective plasma samples were positive. Two patients were positive for HHV-6 antibodies. Since the significance of HHV-6 DNA in PBMCs is unclear, these findings do not necessarily indicate active infection but may be due to mild immunosuppression in autologous BMT recipients.
在早年初次感染后，人类疱疹病毒6型（HHV-6）会潜伏在体内，并可能在免疫状态不佳的个体中重新激活。对23名自体骨髓移植受者进行了一项为期180天的HHV-6感染纵向研究，以评估HHV-6的重新激活情况；其中两名患者接受了两次移植。通过热启动巢式PCR对患者外周血单个核细胞（PBMC）中的HHV-6 DNA进行前瞻性监测。阳性样本通过酶切方案进行分型。对阳性血浆样本也进行了HHV-6 DNA检测。通过免疫荧光法检测针对HHV-6的抗体。23名患者中分别有5名和2名患者的PBMC对HHV-6 DNA呈间歇性和持续性阳性；4名患者携带B型变异株，另外3名患者同时携带A和B型。相应的血浆样本均无阳性结果。两名患者的HHV-6抗体呈阳性。由于PBMC中HHV-6 DNA的意义尚不清楚，这些发现不一定表明存在活动性感染，可能是由于自体骨髓移植受者存在轻度免疫抑制。
