Nitsche A, Müller C W, Radonic A, Landt O, Ellerbrok H, Pauli G, Siegert W
Klinik für Innere Medizin m.S. Hämatologie und Onkologie, Charité-Campus Virchow Klinikum, Humboldt Universität zu Berlin, Berlin, Germany.
J Infect Dis. 2001 Jan 1;183(1):130-3. doi: 10.1086/317651. Epub 2000 Nov 10.
A real-time quantitative polymerase chain reaction assay was devised to determine the load of human herpesvirus (HHV)-6A and -6B DNA in paired samples of plasma and peripheral blood leukocytes (PBL) of 25 bone marrow transplant patients. The assay detects HHV-6 DNA variants A and B in a linear range of 10(7)-10(1) genome equivalents per assay. Viral DNA was measured in 336 paired DNA PBL samples and in corresponding plasma samples. HHV-6A and/or -6B DNA was detected in PBL of 23 of 25 patients and in plasma of 24 of 25 patients. HHV-6B was the predominant variant found in PBL and also was detected in the corresponding plasma. Surprisingly, only 1 of 25 patients had detectable HHV-6A DNA in PBL, although 23 of 25 patients were positive for HHV-6A DNA in plasma. HHV-6 DNA load in plasma was significantly higher for HHV-6A than for HHV-6B (P=.0066).
设计了一种实时定量聚合酶链反应检测方法，以测定25例骨髓移植患者血浆和外周血白细胞（PBL）配对样本中人类疱疹病毒（HHV）-6A和-6B DNA的载量。该检测方法在每次检测10(7)-10(1)个基因组当量的线性范围内检测HHV-6 DNA变异体A和B。在336对DNA PBL样本及其相应血浆样本中测量病毒DNA。在25例患者中的23例PBL以及25例患者中的24例血浆中检测到HHV-6A和/或-6B DNA。HHV-6B是在PBL中发现的主要变异体，在相应血浆中也被检测到。令人惊讶的是，25例患者中只有1例PBL中可检测到HHV-6A DNA，尽管25例患者中有23例血浆中HHV-6A DNA呈阳性。HHV-6A在血浆中的DNA载量显著高于HHV-6B（P=0.0066）。
