Korompilias A V, Chen L E, Seaber A V, Urbaniak J R
Department of Surgery, Duke University Medical Center, Durham, North Carolina 27710, USA.
J Orthop Res. 1999 Sep;17(5):714-9. doi: 10.1002/jor.1100170514.
This study was conducted to elucidate the role of the cytokine interleukin-1 beta on peripheral nerve recovery following crush injuries of two different magnitudes. Eighty-eight female rats were divided into four groups. A 5-mm segment of the right sciatic nerve was subjected to a 100-g crush load for 2 hours in the rats in Groups A1 and B1 or to a 15,000-g crush load for 10 minutes in the rats in Groups A2 and B2. The rats in Groups A1 and A2 received 10 microg/100 g body weight human recombinant interleukin-1 beta intraperitoneally 48, 24, and 1 hours before the nerve injury. The rats in Groups B1 and B2 were treated with an equal volume of normal saline solution with identical schedule guidelines. Walking-track tests (sciatic functional index) performed at intervals until 56 days after the crush and measurements of the contractile force of the extensor digitorum longus muscle made until 28 days were used to evaluate functional recovery of the nerve. During the second week after injury, the rats treated with interleukin-1 beta (A1) had an earlier recovery on the walking track than did those treated with saline solution (B1); this difference reached significance (p < 0.05) at day 11. Although Group A2 demonstrated a trend toward earlier recovery compared with Group B2, there was no significant difference between the two groups. After low or high-load crush injury, tetanic contractile forces were greater in the rats treated with human recombinant interleukin-1 beta than in those treated with saline solution. The results suggest that treatment with human recombinant interleukin-1 beta before crush injury can promote function in the peripheral nerve after the injury. However, the mechanisms that underlie the observed beneficial effects are not completely understood and only speculations can be made.
本研究旨在阐明细胞因子白细胞介素-1β在两种不同程度挤压伤后周围神经恢复中的作用。88只雌性大鼠被分为四组。在A1组和B1组大鼠中，对右侧坐骨神经的5毫米节段施加100克的挤压负荷，持续2小时；在A2组和B2组大鼠中，对右侧坐骨神经的5毫米节段施加15000克的挤压负荷，持续10分钟。A1组和A2组大鼠在神经损伤前48小时、24小时和1小时腹腔注射10微克/100克体重的人重组白细胞介素-1β。B1组和B2组大鼠按照相同的时间表用等量的生理盐水进行处理。每隔一段时间进行步行轨迹测试（坐骨神经功能指数），直至挤压伤后56天，并在28天内测量趾长伸肌的收缩力，以评估神经的功能恢复情况。在损伤后的第二周，接受白细胞介素-1β治疗的大鼠（A1组）在步行轨迹上的恢复比接受生理盐水治疗的大鼠（B1组）更早；这种差异在第11天达到显著水平（p < 0.05）。尽管A2组与B2组相比显示出更早恢复的趋势，但两组之间没有显著差异。在低负荷或高负荷挤压伤后，接受人重组白细胞介素-1β治疗的大鼠的强直收缩力比接受生理盐水治疗的大鼠更大。结果表明，挤压伤前用人重组白细胞介素-1β治疗可促进损伤后周围神经的功能。然而，观察到的有益作用的潜在机制尚未完全了解，只能进行推测。
