De Angelis C, Scarfò C, Falcinelli M, Reda E, Ramacci M T, Angelucci L
Institute for Research on Senescence, Sigma Tau S.p.A., Pomezia, Rome, Italy.
Int J Clin Pharmacol Res. 1992;12(5-6):269-79.
The effects of levocarnitine acetyl were investigated on both peripheral nerve regeneration and neuromuscular remodelling in male Sprague-Dawley rats, three months of age, following crush of their left sciatic nerve. Levocarnitine acetyl, 150 mg/kg/day in drinking water, was given from one week before to 5, 15, 20, and 60 days after nerve crush. The sciatic nerve was examined morphologically at all given times and morphometrically at 15, 20, and 60 days after the lesion. Morphology, at 5, 15, and 60 days, and morphometry, at 60 days after the nerve crush, were also performed on the neuromuscular junction in the soleus and extensor digitorum longus muscles. Five days after nerve crush, complete axonal degeneration was observed in both control and treated rats. At 15 and 20 days, recovery from injury in treated animals was better than in controls, as shown by a significantly higher increase in the number of regenerating axons. At the same times, denervated endplates were present in both groups. At 60 days, axonal regeneration restored the number of axons to normal values in all injured animals, while their size maturation was greater in treated rats than in controls. A markedly lower number of degenerating elements was found in treated animals. In the neuromuscular junctions of the soleus and extensor digitorum longus muscles, nerve terminal branch points were reduced in the lesioned rats in comparison with uninjured ones. However, morphometric analysis revealed a greater endplate complexity in treated animals in which, at 60 days after nerve crush, nerve terminal branching and sprouting index values were significantly higher than in controls. It is concluded that levocarnitine acetyl exerts a beneficial effect on nerve regeneration processes and synaptic remodelling in crush-induced neuropathies.
研究了左旋肉碱乙酰酯对3月龄雄性Sprague-Dawley大鼠左侧坐骨神经挤压伤后周围神经再生和神经肌肉重塑的影响。从神经挤压伤前1周开始，至伤后5、15、20和60天，给予大鼠含150 mg/kg/天左旋肉碱乙酰酯的饮用水。在所有给定时间对坐骨神经进行形态学检查，并在损伤后15、20和60天进行形态计量学分析。在比目鱼肌和趾长伸肌的神经肌肉接头处，也于神经挤压伤后5、15和60天进行形态学检查，60天时进行形态计量学分析。神经挤压伤5天后，在对照大鼠和治疗大鼠中均观察到完全性轴突退变。在15和20天时，治疗组动物的损伤恢复情况优于对照组，表现为再生轴突数量显著增加。同时，两组均存在失神经终板。60天时，轴突再生使所有损伤动物的轴突数量恢复至正常水平，而治疗组大鼠轴突的大小成熟度高于对照组。治疗组动物中退变成分的数量明显减少。在比目鱼肌和趾长伸肌的神经肌肉接头处，与未损伤大鼠相比，损伤大鼠的神经终末分支点减少。然而，形态计量学分析显示，治疗组动物的终板复杂性更高，在神经挤压伤60天时，其神经终末分支和发芽指数值显著高于对照组。结论是，左旋肉碱乙酰酯对挤压伤所致神经病变的神经再生过程和突触重塑具有有益作用。
