Stackhouse G B, Sesterhenn I A, Bauer J J, Mostofi F K, Connelly R R, Srivastava S K, Moul J W
Department of Surgery, Walter Reed Army Medical Center, Washington, DC, USA.
J Urol. 1999 Dec;162(6):2040-5. doi: 10.1016/S0022-5347(05)68095-0.
Immunohistochemical staining of radical prostatectomy specimens for p53 and bcl-2 proteins has been shown to correlate with prostate specific antigen (PSA) recurrence in a series of patients at our institution. We analyzed the relationship between staining of diagnostic prostate needle biopsies for p53 and bcl-2, and PSA recurrence.
From 1986 to 1993, 335 radical prostatectomies were performed at our hospital. Of the prostatectomy specimens 199 had been evaluated for p53 and bcl-2 proteins in a prior series. Of 139 patients with biopsy material available for analysis 129 had enough for evaluation of 1 or both markers. Prospectively obtained clinical followup data were available, with a mean followup of 6 years. Commercially available antibodies were used for immunohistochemical staining.
The overall PSA recurrence rate was 37.6% for 199 radical prostatectomy cases and 37.9% for 129 with biopsy immunohistochemical staining. Staining of prostatectomies correlated well with PSA recurrence for p53 (p = 0.004) and bcl-2 (p = 0.001). However, biopsy staining did not correlate with prostatectomy staining or PSA recurrence for either marker.
The p53 and bcl-2 biomarkers appear to be important to predict recurrence of prostate cancer when prostatectomy specimens are analyzed but this usefulness is not apparent with immunohistochemical staining of prostate biopsies. This difference may reflect sampling error and/or the heterogeneous nature of prostate cancers, and deserves further study.
在我们机构的一系列患者中，前列腺癌根治术标本的p53和bcl-2蛋白免疫组化染色已显示与前列腺特异性抗原（PSA）复发相关。我们分析了诊断性前列腺穿刺活检中p53和bcl-2染色与PSA复发之间的关系。
1986年至1993年，我院共进行了335例前列腺癌根治术。在之前的一项研究中，对199例前列腺癌根治术标本进行了p53和bcl-2蛋白评估。在139例有活检材料可供分析的患者中，129例有足够材料评估1种或两种标志物。可获得前瞻性的临床随访数据，平均随访时间为6年。使用市售抗体进行免疫组化染色。
199例前列腺癌根治术病例的总体PSA复发率为37.6%，129例有活检免疫组化染色的病例为37.9%。前列腺癌根治术标本的p53（p = 0.004）和bcl-2（p = 0.001）染色与PSA复发密切相关。然而，对于这两种标志物，活检染色与前列腺癌根治术标本染色或PSA复发均无相关性。
在分析前列腺癌根治术标本时，p53和bcl-2生物标志物似乎对预测前列腺癌复发很重要，但在前列腺活检的免疫组化染色中这种作用并不明显。这种差异可能反映了抽样误差和/或前列腺癌的异质性，值得进一步研究。
