Hussain M, Peters G, Chhatwal G S, Herrmann M
Institute of Medical Microbiology, University Hospital of Münster, Münster, Germany.
Infect Immun. 1999 Dec;67(12):6688-90. doi: 10.1128/IAI.67.12.6688-6690.1999.
To identify novel putative staphylococcal adhesins, lithium chloride extraction (an established method for selective surface molecule solubilization) was employed. N-terminal sequencing and functional assays identified a 42-kDa fibronectin-binding protein from Staphylococcus epidermidis as ornithine carbamoyltransferase (OCTase). However, OCTase was not recognizable extracellularly, and this fact together with the fact that LiCl induced DNA release and a decrease in viability suggests that LiCl extraction may not be the method of choice for selective surface molecule extraction from staphylococci.
为了鉴定新型的假定葡萄球菌黏附素,采用了氯化锂提取法(一种用于选择性溶解表面分子的既定方法)。N端测序和功能分析确定了表皮葡萄球菌中一种42 kDa的纤连蛋白结合蛋白为鸟氨酸氨甲酰基转移酶(OCTase)。然而,OCTase在细胞外无法识别,并且氯化锂诱导DNA释放以及活力下降这一事实表明,氯化锂提取法可能不是从葡萄球菌中选择性提取表面分子的首选方法。