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本文引用的文献

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Surface protein adhesins of Staphylococcus aureus.金黄色葡萄球菌的表面蛋白黏附素
Trends Microbiol. 1998 Dec;6(12):484-8. doi: 10.1016/s0966-842x(98)01400-0.
2
The staphylococcal transferrin-binding protein is a cell wall glyceraldehyde-3-phosphate dehydrogenase.葡萄球菌转铁蛋白结合蛋白是一种细胞壁甘油醛-3-磷酸脱氢酶。
Infect Immun. 1999 Mar;67(3):1086-92. doi: 10.1128/IAI.67.3.1086-1092.1999.
3
A fibrinogen-binding protein of Staphylococcus epidermidis.表皮葡萄球菌的一种纤维蛋白原结合蛋白。
Infect Immun. 1998 Jun;66(6):2666-73. doi: 10.1128/IAI.66.6.2666-2673.1998.
4
The glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase of Candida albicans is a surface antigen.白色念珠菌的糖酵解酶3-磷酸甘油醛脱氢酶是一种表面抗原。
J Bacteriol. 1997 Aug;179(16):4992-9. doi: 10.1128/jb.179.16.4992-4999.1997.
5
Evidence for autolysin-mediated primary attachment of Staphylococcus epidermidis to a polystyrene surface.表皮葡萄球菌自溶素介导其与聚苯乙烯表面发生初始黏附的证据。
Mol Microbiol. 1997 Jun;24(5):1013-24. doi: 10.1046/j.1365-2958.1997.4101774.x.
6
Characterization of Tn917 insertion mutants of Staphylococcus epidermidis affected in biofilm formation.表皮葡萄球菌生物膜形成受影响的Tn917插入突变体的特性分析。
Infect Immun. 1996 Jan;64(1):277-82. doi: 10.1128/iai.64.1.277-282.1996.
7
Identification of a Staphylococcus aureus extracellular matrix-binding protein with broad specificity.鉴定一种具有广泛特异性的金黄色葡萄球菌细胞外基质结合蛋白。
Infect Immun. 1993 Jun;61(6):2479-85. doi: 10.1128/iai.61.6.2479-2485.1993.
8
Purification of a bone sialoprotein-binding protein from Staphylococcus aureus.从金黄色葡萄球菌中纯化一种骨唾液蛋白结合蛋白。
Eur J Biochem. 1994 Jun 15;222(3):919-25. doi: 10.1111/j.1432-1033.1994.tb18940.x.
9
MSCRAMM-mediated adherence of microorganisms to host tissues.微生物表面成分识别黏附分子介导的微生物与宿主组织的黏附
Annu Rev Microbiol. 1994;48:585-617. doi: 10.1146/annurev.mi.48.100194.003101.
10
Two novel cationic staphylococcal proteins induce IL-2 secretion, proliferation and immunoglobulin synthesis in peripheral blood mononuclear cells (PBMC) of both healthy controls and patients with common variable immunodeficiency (CVID).两种新型阳离子葡萄球菌蛋白可诱导健康对照者和常见变异型免疫缺陷(CVID)患者外周血单核细胞(PBMC)分泌白细胞介素-2、增殖并合成免疫球蛋白。
Clin Exp Immunol. 1995 Jun;100(3):406-11. doi: 10.1111/j.1365-2249.1995.tb03714.x.

表皮葡萄球菌的一种经氯化锂提取的、广谱黏附性42千道尔顿蛋白质是鸟氨酸氨甲酰基转移酶。

A lithium chloride-extracted, broad-spectrum-adhesive 42-kilodalton protein of Staphylococcus epidermidis is ornithine carbamoyltransferase.

作者信息

Hussain M, Peters G, Chhatwal G S, Herrmann M

机构信息

Institute of Medical Microbiology, University Hospital of Münster, Münster, Germany.

出版信息

Infect Immun. 1999 Dec;67(12):6688-90. doi: 10.1128/IAI.67.12.6688-6690.1999.

DOI:10.1128/IAI.67.12.6688-6690.1999
PMID:10569792
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC97084/
Abstract

To identify novel putative staphylococcal adhesins, lithium chloride extraction (an established method for selective surface molecule solubilization) was employed. N-terminal sequencing and functional assays identified a 42-kDa fibronectin-binding protein from Staphylococcus epidermidis as ornithine carbamoyltransferase (OCTase). However, OCTase was not recognizable extracellularly, and this fact together with the fact that LiCl induced DNA release and a decrease in viability suggests that LiCl extraction may not be the method of choice for selective surface molecule extraction from staphylococci.

摘要

为了鉴定新型的假定葡萄球菌黏附素,采用了氯化锂提取法(一种用于选择性溶解表面分子的既定方法)。N端测序和功能分析确定了表皮葡萄球菌中一种42 kDa的纤连蛋白结合蛋白为鸟氨酸氨甲酰基转移酶(OCTase)。然而,OCTase在细胞外无法识别,并且氯化锂诱导DNA释放以及活力下降这一事实表明,氯化锂提取法可能不是从葡萄球菌中选择性提取表面分子的首选方法。