Spencer T E, Gray A, Johnson G A, Taylor K M, Gertler A, Gootwine E, Ott T L, Bazer F W
Center for Animal Biotechnology and Genomics, Texas A&M University System Health Science Center, and Department of Animal Science, Texas A&M University, College Station 77843-2471, USA.
Biol Reprod. 1999 Dec;61(6):1409-18. doi: 10.1095/biolreprod61.6.1409.
Studies were conducted to determine effects of intrauterine administration of recombinant ovine interferon tau (IFNtau), placental lactogen (PL), and growth hormone (GH) on endometrial function. In the first study, administration of IFNtau to cyclic ewes for one period (Days 11-15) resulted in an interestrous interval (IEI) of approximately 30 days, whereas administration for two periods (Days 11-15 and Days 21-25) extended the IEI to greater than 50 days. Administration of IFNtau from Days 11 to 15 and of PL or GH from Days 21 to 25 failed to extend the IEI more than for IFNtau alone. In the second study, effects of IFNtau, PL, and GH on endometrial differentiation and function were determined in ovariectomized ewes receiving ovarian steroid replacement therapy. Endometrial expression of mRNAs for estrogen receptor (ER), progesterone receptor (PR), and oxytocin receptor (OTR) were not affected by PL or GH treatment; however, uterine milk protein mRNA levels and stratum spongiosum gland density were increased by both PL and GH treatments. Collectively, results indicated that 1) PL and GH do not regulate endometrial PR, ER, and OTR expression or affect corpus luteum life span; 2) down-regulation of epithelial PR expression is requisite for progesterone induction of secretory gene expression in uterine glandular epithelium; 3) effects of PL and GH on endometrial function require IFNtau; and 4) PL and GH regulate endometrial gland proliferation and perhaps differentiated function.
开展了多项研究，以确定子宫内注射重组羊干扰素τ（IFNτ）、胎盘催乳素（PL）和生长激素（GH）对子宫内膜功能的影响。在第一项研究中，给处于发情周期的母羊在一个时期（第11 - 15天）注射IFNτ，导致发情间期（IEI）约为30天，而在两个时期（第11 - 15天和第21 - 25天）注射则使IEI延长至超过50天。在第11至15天注射IFNτ，在第21至25天注射PL或GH，未能使IEI延长超过单独注射IFNτ的情况。在第二项研究中，在接受卵巢类固醇替代疗法的去卵巢母羊中确定了IFNτ、PL和GH对子宫内膜分化和功能的影响。雌激素受体（ER）、孕激素受体（PR）和催产素受体（OTR）的mRNA在子宫内膜中的表达不受PL或GH治疗的影响；然而，PL和GH治疗均使子宫乳蛋白mRNA水平和海绵层腺密度增加。总体而言，结果表明：1）PL和GH不调节子宫内膜PR、ER和OTR的表达，也不影响黄体寿命；2）上皮PR表达的下调是孕激素诱导子宫腺上皮分泌基因表达所必需的；3）PL和GH对子宫内膜功能的影响需要IFNτ；4）PL和GH调节子宫内膜腺体增殖以及可能的分化功能。
