烟草中编码腈水解酶样蛋白的TNIT4基因的结构分析。

Structural analysis of the TNIT4 genes encoding nitrilase-like protein from tobacco.

作者信息

Dohmoto M, Sano J, Tsunoda H, Yamaguchi K

机构信息

Institute for Gene Research, Kanazawa University, Japan.

出版信息

DNA Res. 1999 Oct 29;6(5):313-7. doi: 10.1093/dnares/6.5.313.

DOI:10.1093/dnares/6.5.313

PMID:10574458

Abstract

Nitrilase (nitrile aminohydrolase, EC 3.5.5.1) catalyzes the hydrolysis of indole-3-acetonitrile (IAN) to indole-3-acetic acid (IAA). The Arabidopsis thaliana genome has four nitrilase genes (NIT1 to NIT4), while tobacco (Nicotiana tabacum) has only NIT4 homologs (TNIT4A and TNIT4B) and no NIT1 to NIT3 homologs. We have cloned the promoter region of TNIT4B and determined the transcriptional start sites which are the same sites in both TNIT4A and TNIT4B. The TNIT4 genes are expressed in various organs at low levels. The positions of the exon-intron splicing junctions in Arabidopsis NIT1 to NIT3 are completely conserved in TNIT4A.

摘要

腈水解酶（腈氨基水解酶，EC 3.5.5.1）催化吲哚-3-乙腈（IAN）水解生成吲哚-3-乙酸（IAA）。拟南芥基因组有四个腈水解酶基因（NIT1至NIT4），而烟草（烟草）只有NIT4同源物（TNIT4A和TNIT4B），没有NIT1至NIT3同源物。我们克隆了TNIT4B的启动子区域，并确定了转录起始位点，这些位点在TNIT4A和TNIT4B中是相同的。TNIT4基因在各个器官中低水平表达。拟南芥NIT1至NIT3中外显子-内含子剪接位点的位置在TNIT4A中完全保守。