Hillebrand H, Tiemann B, Hell R, Bartling D, Weiler E W
Lehrstuhl für Pflanzenphysiologie, Ruhr-Universität, Bochum, Germany.
Gene. 1996 May 8;170(2):197-200. doi: 10.1016/0378-1119(95)00839-x.
The nitrilases of Arabidopsis thaliana (At) catalyze the conversion of indole-3-acetonitrile (IAN) to indole-3-acetic acid (IAA), thus controlling the last step of auxin biosynthesis. A full-length genomic clone encoding the complete cluster of the At nitrilases 1 to 3 (NIT1-3), including the respective promoter regions, has been isolated and the NIT1 isoform has been sequenced. The coding region (nit1) spans about 2.3 kb and is composed of five exons separated by four introns. The exon-intron splice junctions agree with the consensus sequences typical for plant genes. In agreement with the known cDNA sequence, the exons encode a protein of 346 amino acids (aa) with a deduced molecular mass of 38.2 kDa. The transcription start point (tsp) of nit1 was determined by primer extension experiments. This tsp defines a 5' untranslated region of 36 bp and is located 32 bp downstream from a TATA box. The promoter region of nit1 is located within the approx. 1.5-kb intergenic part that separates the nit2 and nit1 coding sections.
拟南芥（At）的腈水解酶催化吲哚 - 3 - 乙腈（IAN）转化为吲哚 - 3 - 乙酸（IAA），从而控制生长素生物合成的最后一步。已分离出一个编码At腈水解酶1至3（NIT1 - 3）完整簇的全长基因组克隆，包括各自的启动子区域，并对NIT1同工型进行了测序。编码区（nit1）跨度约2.3 kb，由五个外显子和四个内含子分隔。外显子 - 内含子剪接接头与植物基因典型的共有序列一致。与已知的cDNA序列一致，外显子编码一个346个氨基酸（aa）的蛋白质，推导分子量为38.2 kDa。通过引物延伸实验确定了nit1的转录起始点（tsp）。该tsp定义了一个36 bp的5'非翻译区，位于TATA框下游32 bp处。nit1的启动子区域位于分隔nit2和nit1编码区的约1.5 kb基因间隔区内。
