Fujinaga K, Sekikawa K, Yamazaki H, Green M
Cold Spring Harb Symp Quant Biol. 1975;39 Pt 1:633-6. doi: 10.1101/sqb.1974.039.01.076.
The presence of multiple copies of a portion of the viral genome was demonstrated in Ad7-transformed cells by a new method based on the kinetic analysis of labeled viral DNA reassociation in the presence of unlabeled transformed cell DNA. DNA-DNA homology measurement utilizing defined DNA fragments (restriction endonuclease digests) as labeled probes supported the above conclusion.
通过一种基于在未标记的转化细胞DNA存在下对标记病毒DNA重缔合进行动力学分析的新方法,在腺病毒7型转化细胞中证实了病毒基因组一部分的多个拷贝的存在。利用特定DNA片段(限制性内切酶消化产物)作为标记探针进行的DNA-DNA同源性测量支持了上述结论。