Lancaster W D, Olson C, Meinke W
J Virol. 1976 Mar;17(3):824-31. doi: 10.1128/JVI.17.3.824-831.1976.
Bovine papilloma virus (BPV) DNA was labeled in vitro under conditions of repair synthesis and subsequently used as a "probe" in DNA-DNA reassociation studies to detect BPV-specific DNA sequences in a viral-induced calf meningioma and hamster fibroma. In vitro labeled BPV DNA had denaturation characteristics expected for duplex DNA and denatured DNA reassociated with apparent second-order kinetics. Analysis of in vitro labeled BPV DNA reassociation rates in the presence of excess tumor DNA revealed that the calf meningioma contained approximately 700 to 800 BPV genome equivalents per diploid cell whereas the hamster fibroma contained about 150 incomplete BPV genome equivalents per diploid cell. Thermal denaturation of in vitro labeled BPV DNA which reassociated in the presence of the two tumor DNA preparations indicated less than 1.5% base pair mismatching.
牛乳头瘤病毒（BPV）DNA在修复合成条件下进行体外标记，随后用作DNA-DNA重缔合研究中的“探针”，以检测病毒诱导的小牛脑膜瘤和仓鼠纤维瘤中的BPV特异性DNA序列。体外标记的BPV DNA具有双链DNA预期的变性特征，变性DNA以明显的二级动力学进行重缔合。在过量肿瘤DNA存在的情况下对体外标记的BPV DNA重缔合速率进行分析，结果显示小牛脑膜瘤每个二倍体细胞含有约700至800个BPV基因组当量，而仓鼠纤维瘤每个二倍体细胞含有约150个不完整的BPV基因组当量。在两种肿瘤DNA制剂存在下重缔合的体外标记BPV DNA的热变性表明碱基对错配小于1.5%。
