对嗜热古菌嗜酸热硫化叶菌23S核糖体RNA中假尿苷残基的首次测定。

The first determination of pseudouridine residues in 23S ribosomal RNA from hyperthermophilic Archaea Sulfolobus acidocaldarius.

作者信息

Massenet S, Ansmant I, Motorin Y, Branlant C

机构信息

Laboratoire de Maturation des ARN et Enzymologie Moléculaire, UMR 7567 CNRS-UHP Nancy I, Faculté des Sciences, P.O. Box 239, 54506, Vandoeuvre-les-Nancy, France.

出版信息

FEBS Lett. 1999 Nov 26;462(1-2):94-100. doi: 10.1016/s0014-5793(99)01524-0.

DOI:10.1016/s0014-5793(99)01524-0

PMID:10580099

Abstract

We describe the first identification of pseudouridine (Psi) residues in ribosomal RNA (23S rRNA) of an hyperthermophilic Archaea Sulfolobus acidocaldarius. In contrast to Eucarya rRNA, only six Psi residues were detected, which is rather close to the situation in Bacteria. However, three modified positions (Psi(2479), Psi(2535) and Psi(2550)) are unique for S. acidocaldarius. Two Psi residues at positions 2060 and 2594 are universally conserved, while one other Psi (position 2066) is also common to Eucarya. Taken together the results argue against the conservation of Psi-synthases between Archaea and Bacteria and provide a basis for the search of snoRNA-like guides for Psi formation in Archaea.

摘要

我们描述了首次在嗜热古菌嗜酸热硫化叶菌的核糖体RNA（23S rRNA）中鉴定出假尿苷（Ψ）残基。与真核生物rRNA不同，仅检测到六个Ψ残基，这与细菌中的情况相当接近。然而，三个修饰位点（Ψ(2479)、Ψ(2535)和Ψ(2550)）是嗜酸热硫化叶菌所特有的。2060位和2594位的两个Ψ残基是普遍保守的，而另一个Ψ（2066位）在真核生物中也很常见。综合这些结果表明，古菌和细菌之间不存在保守的Ψ合成酶，并为寻找古菌中形成Ψ的类snoRNA向导提供了基础。