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嗜热古菌嗜酸热硫化叶菌的菌株鉴定及5S rRNA基因特征分析

Strain identification and 5S rRNA gene characterization of the hyperthermophilic archaebacterium Sulfolobus acidocaldarius.

作者信息

Durovic P, Kutay U, Schleper C, Dennis P P

机构信息

Department of Biochemistry and Molecular Biology and Canadian Institute for Advanced Research, University of British Columbia, Vancouver, Canada.

出版信息

J Bacteriol. 1994 Jan;176(2):514-7. doi: 10.1128/jb.176.2.514-517.1994.

DOI:10.1128/jb.176.2.514-517.1994
PMID:8288546
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC205076/
Abstract

A commonly used laboratory Sulfolobus strain has been unambiguously identified as Sulfolobus acidocaldarius DSM639. The 5S rRNA gene from this strain was cloned and sequenced. It differs at 17 of 124 positions from the identical 5S rRNA sequences from Sulfolobus solfataricus and a strain apparently misidentified as S. acidocaldarius. Analysis of the transcripts from the 5S rRNA gene failed to identify any precursor extending a significant distance beyond the 5' or 3' boundary of the 5S rRNA-coding sequence. This result suggests that the primary transcript of the 5S rRNA gene corresponds in length (within 1 or 2 nucleotides) to the mature 5S rRNA sequence found in 50S ribosomal subunits.

摘要

一种常用的实验室嗜硫珠菌菌株已被明确鉴定为嗜酸嗜热栖热菌DSM639。克隆并测序了该菌株的5S rRNA基因。它与嗜酸热硫化叶菌以及一株明显被误鉴定为嗜酸嗜热栖热菌的菌株的相同5S rRNA序列相比，在124个位置中有17个位置不同。对5S rRNA基因转录本的分析未能鉴定出任何在5S rRNA编码序列的5'或3'边界之外有显著延伸的前体。这一结果表明，5S rRNA基因的初级转录本长度（在1或2个核苷酸范围内）与在50S核糖体亚基中发现的成熟5S rRNA序列相对应。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/345b/205076/6d1c7793ac28/jbacter00020-0260-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/345b/205076/6d1c7793ac28/jbacter00020-0260-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/345b/205076/6d1c7793ac28/jbacter00020-0260-a.jpg

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本文引用的文献

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