Challacombe S J, Russell M W, Hawkes J
Clin Exp Immunol. 1978 Dec;34(3):417-22.
The aim of this investigation was to determine whether IgG could pass from the blood to the oral cavity. Pure IgG was prepared from monkey serum, by ion exchange chromatography and gel filtration, and was radiolabelled with I. This was injected intravenously into eight Rhesus monkeys. Radioactivity could be detected in crevicular fluid washings, and in mixed and parotid saliva samples 30 min after injection. Ultracentrifugation on sucrose density gradients revealed that most of the radioactivity in crevicular fluid washings was associated with proteins having a sedimentation coefficient similar to marker IgG. Radioactivity in parotid saliva was not found in the IgG zone, but was present in zones with sedimentation coefficients of approximately 4·5S and 1S. The results suggest that IgG passes as an intact molecule from plasma to crevicular fluid, and support the hypothesis that serum antibodies could play a role in protection against dental caries.
本研究的目的是确定IgG是否能从血液进入口腔。通过离子交换色谱法和凝胶过滤法从猴血清中制备了纯IgG，并用碘进行放射性标记。将其静脉注射到8只恒河猴体内。注射后30分钟，在龈沟液冲洗液、混合唾液和腮腺唾液样本中可检测到放射性。在蔗糖密度梯度上进行超速离心显示，龈沟液冲洗液中的大部分放射性与沉降系数与标记IgG相似的蛋白质相关。腮腺唾液中的放射性在IgG区未发现，但存在于沉降系数约为4.5S和1S的区域。结果表明，IgG作为完整分子从血浆进入龈沟液，并支持血清抗体可能在预防龋齿中发挥作用的假说。
