鸡横纹肌的体外肌生成：胶原蛋白在肌纤维形成中的作用。
Myogenesis of avian striated muscle in vitro: role of collagen in myofiber formation.
作者信息
De la Haba G, Kamali H M, Tiede D M
出版信息
Proc Natl Acad Sci U S A. 1975 Jul;72(7):2729-32. doi: 10.1073/pnas.72.7.2729.
The complex chemical environment required for the development of the myofiber (myotube) from embryonic avian muscle myoblasts in vitro has been simplified. Myotube formation is shown to occur in the presence of insulin, a low-molecular-weight (fusion) factor obtained from embryo extract, and a collagen substratum. In the absence of collagen, globular structures are formed which are microscopically identical to the globular syncytial structures previously described [de la Haba and Amundsen (1972) Proc. Nat. Acad. Sci. USA 69, 1131-1135]. A high-molecular-weight fraction from embryo extract will also promote myotube formation in the presence of the fusion factor. This fraction is shown to contain (a) collagen, which promotes myotube formation, and (b) an additional factor which also promotes myotube formation, and which we tentatively hypothsize to be an inducer of collagen synthesis by myogenic cells.
体外培养鸡胚胎肌肉成肌细胞以形成肌纤维（肌管）所需的复杂化学环境已得到简化。研究表明，在胰岛素、一种从胚胎提取物中获得的低分子量（融合）因子以及胶原基质存在的情况下，肌管能够形成。在没有胶原的情况下，会形成球状结构，在显微镜下，这些结构与之前描述的球状多核体结构相同[德拉·哈巴和阿蒙森（1972年），《美国国家科学院院刊》69卷，第1131 - 1135页]。在融合因子存在的情况下，胚胎提取物中的高分子量组分也会促进肌管形成。该组分被证明含有：（a）促进肌管形成的胶原，以及（b）另一种也能促进肌管形成的因子，我们初步推测它是成肌细胞合成胶原的诱导剂。
Zotero 插件