Mauri P, Migliazza B, Pietta P
ITBA/CNR, Via Fratelli Cervi 93, 20090 Segrate (Milan), Italy.
J Mass Spectrom. 1999 Dec;34(12):1361-7. doi: 10.1002/(SICI)1096-9888(199912)34:12<1361::AID-JMS895>3.0.CO;2-6.
Standardized extracts of Ginkgo biloba leaves are mainly used in the treatment of peripheral and celebral circulation disorders, and also as a remedy against asthma, coughs, bladder inflammation, blenorrhagia and alcohol abuse. The leaf extracts contain biflavones, flavonol glycosides and terpene lactones. This paper reports a method based on liquid chromatography coupled with electrospray mass spectrometry for the analysis of terpenoids in G. biloba extracts. This method allows the rapid isocratic separation of underivatized ginkgolides (GA, GB, GC and GJ) and bilobalide at very low levels (10 pg on the column) and their quantitative detection by external standardization with relative standard deviations of 3 and 5% for intra- and inter-day analyses, respectively.
银杏叶标准化提取物主要用于治疗外周和脑部循环紊乱，也用作治疗哮喘、咳嗽、膀胱炎、白带过多及酒精滥用的药物。叶提取物含有双黄酮、黄酮醇苷和萜类内酯。本文报道了一种基于液相色谱-电喷雾质谱联用的方法，用于分析银杏提取物中的萜类化合物。该方法能在非常低的水平（柱上10皮克）对未衍生化的银杏内酯（GA、GB、GC和GJ）和白果内酯进行快速等度分离，并通过外标法进行定量检测，日内和日间分析的相对标准偏差分别为3%和5%。
