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测序作为酵母分子分类学中的一种工具。

Sequencing as a tool in yeast molecular taxonomy.

作者信息

Valente P, Ramos J P, Leoncini O

机构信息

Departamento de Genética, Universidade Federal do Rio de Janeiro, Ilha do Fundão, Brasil.

出版信息

Can J Microbiol. 1999 Nov;45(11):949-58. doi: 10.1139/w99-094.

Abstract

The literature on sequencing as a tool for yeast molecular taxonomy is reviewed. Ribosomal DNA has been preferred for sequencing over other molecules such as mitochondrial DNA, and a large database is now available. rDNA consists of regions that evolve at different rates, allowing comparison of different levels of relationship among yeasts. Sequences of the 18S rDNA and the 25S rDNA have been largely used for yeast systematics and phylogeny, but the search for regions with increased resolving power has led to the study of the spacer regions of the rDNA. Few studies are concerned with signature sequences.

摘要

本文综述了将测序作为酵母分子分类学工具的相关文献。与线粒体DNA等其他分子相比,核糖体DNA更适合用于测序,目前已有一个大型数据库。核糖体DNA由进化速率不同的区域组成,这使得能够比较酵母之间不同层次的亲缘关系。18S核糖体DNA和25S核糖体DNA的序列在很大程度上已被用于酵母系统学和系统发育研究,但对具有更高分辨率区域的探索促使人们对核糖体DNA的间隔区进行研究。很少有研究关注特征序列。

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