Yu G H, De Frias D V, Horcher A M
Department of Pathology, Northwestern Memorial Hospital, Chicago, IL, USA.
Cytopathology. 1999 Oct;10(5):298-302. doi: 10.1046/j.1365-2303.1999.00200.x.
We have studied 25 serous effusions containing definitive morphologic evidence of adenocarcinoma to evaluate the ability of two mucin stains (Mayer's mucicarmine, D-PAS) to detect intracytoplasmic mucin in both cytologic (cytospin) and corresponding histological (cell block) preparations. Mucicarmine stain was positive in six of 25 (24%) cytospins and 13 of 25 (52%) cell blocks. D-PAS was positive in 19 of 25 (76%) cytospins and 20 of 25 (80%) cell blocks. Eight cases were identified which showed mucicarmine positivity in the cell block but not the corresponding cytospin; prolonging incubation time resulted in a positive mucicarmine in cytospin preparations for seven of these cases. We conclude that: (i) D-PAS is a more sensitive stain for the detection of intracytoplasmic mucin in all preparations; (ii) mucicarmine shows preferential staining for cell blocks; (iii) alterations in the staining protocol may permit mucin detection by mucicarmine staining in cytologic preparations in a significant number of cases.
我们研究了25例含有腺癌明确形态学证据的浆液性积液，以评估两种黏液染色法（迈耶氏黏蛋白胭脂红染色、D-PAS染色）在细胞学（细胞离心涂片）和相应组织学（细胞块）标本中检测胞浆内黏液的能力。在25例细胞离心涂片中，黏蛋白胭脂红染色阳性的有6例（24%），在25例细胞块中有13例（52%）阳性。D-PAS染色在25例细胞离心涂片中19例（76%）阳性，在25例细胞块中有20例（80%）阳性。发现8例在细胞块中黏蛋白胭脂红染色阳性，但相应的细胞离心涂片阴性；延长孵育时间后，其中7例细胞离心涂片标本中黏蛋白胭脂红染色呈阳性。我们得出以下结论：（i）在所有标本中，D-PAS是检测胞浆内黏液更敏感的染色方法；（ii）黏蛋白胭脂红对细胞块显示出优先染色；（iii）在大量病例中，改变染色方案可能使细胞学标本中通过黏蛋白胭脂红染色检测到黏液。
