Yu G H, Sack M J, Baloch Z W, DeFrias D V, Gupta P K
Department of Pathology, Hospital of the University of Pennsylvania, Philadelphia 19104, USA.
Diagn Cytopathol. 1999 Mar;20(3):115-9. doi: 10.1002/(sici)1097-0339(199903)20:3<115::aid-dc1>3.0.co;2-3.
The application of cytomorphologic criteria to the examination of serous effusions allows the reliable diagnosis of malignancy in the majority of cases. One feature observed in tissue fragments previously thought to be indicative of mesothelial origin is the presence of intercellular windows, presumably due to long surface microvilli. In this study, however, we examined cytologic preparations of 143 effusion and body-cavity washing specimens and noted distinct intercellular window formation within tissue fragments of adenocarcinoma in 13% of the cases studied. Stains for mucicarmine, Alcian blue with hyaluronidase pretreatment, and periodic acid-Schiff following diastase digestion on corresponding cell block material demonstrated that intercellular mucin contributes to such window formation in greater than half of these cases. Thus the presence of intercellular windows within tissue fragments does not, in isolation, preclude the diagnosis of malignancy in serous effusions.
将细胞形态学标准应用于浆液性积液检查，在大多数情况下能够可靠地诊断恶性肿瘤。在先前认为提示间皮起源的组织碎片中观察到的一个特征是存在细胞间窗，推测这是由于长表面微绒毛所致。然而，在本研究中，我们检查了143例积液和体腔冲洗标本的细胞学涂片，发现在13%的研究病例中，腺癌组织碎片内有明显的细胞间窗形成。对相应细胞块材料进行黏液卡红染色、透明质酸酶预处理后的阿尔辛蓝染色以及淀粉酶消化后的过碘酸希夫染色显示，在超过一半的此类病例中，细胞间黏液促成了这种窗的形成。因此，组织碎片内细胞间窗的存在本身并不排除浆液性积液中恶性肿瘤的诊断。
